Related Experiment Video
Updated: May 27, 2026

Exploring the Longissimus Muscle: Unraveling its Correlation with Meat Quality in Bos indicus and Crossbred Bulls
Published on: July 12, 2024
Evaluation of Longissimus dorsi muscle pH at three hours Post mortem as a predictor of beef tenderness
S D Shackelford1, M Koohmaraie, J W Savell
1Department of Animal Science, Meat Science Section Texas Agricultural Experiment Station, Texas A & M University, College Station, Texas 77843-2471, USA.
Abstract:
The objective of this study was to determine the relationship of beef longissimus dorsi muscle (LM) pH at 3 h post mortem (pH(3)) and aged LM tenderness. The cattle (n = 444) sampled for this experiment represented various breed types, sex classes, feeding regimes, and post-mortem handling practices. The phenotypic diversity of the cattle used provided a great amount of variation in Warner-Bratzler shear (WBS) force (Coefficient of variation for pH, temperature, and sarcomere length measurements were much smaller than the coefficient of variation for WBS force). None of the parameters measured (LM pH at 3 and 48 h post mortem, temperature at 3 h post mortem and sarcomere length) was strongly related to tenderness. These results do not support the use of pH(3) as a criterion for sorting beef carcasses into expected tenderness groups.

