Related Experiment Video
Updated: May 27, 2026

09:07
The Effect of the Application of Thyme Essential Oil on Microbial Load During Meat Drying
Published on: March 14, 2018
Microbiological examination of pre-storage and post-storage intervention beef
1Food Studies Group, Department of Hotel and Catering Management, University of Ulster at Jordanstown, County Antrim, BT37 0QB, UK.
Meat Science
|November 9, 2011
Summary
Intervention processing of beef resulted in bacterial populations similar to traditional methods. Despite processing differences, pathogen-containing genera remained prevalent in the final product.
Area of Science:
- Food microbiology
- Meat science
- Food safety
Background:
- Intervention processing for beef utilizes novel techniques distinct from traditional carcass dressing.
- Understanding the microbiological implications of these new methods is crucial for ensuring food safety.
- Previous research has not fully characterized the bacterial flora of intervention-processed beef.
Purpose of the Study:
- To compare the microbiological profile of intervention-processed beef with conventionally processed beef.
- To assess the bacterial load and genus diversity in intervention beef before and after storage.
- To identify potential food safety concerns associated with intervention processing.
Main Methods:
- Microbiological analysis of bacterial flora in intervention beef samples.
- Enumeration of bacterial counts (log(10) per gram) under various conditions.
- Assessment of bacterial genus diversity in pre- and post-storage samples.
- Comparison of microbial data with that of chill-stored carcass beef.
Main Results:
- Intervention beef exhibited bacterial populations similar in magnitude to chill-stored carcass beef.
- Mean log(10) bacterial counts ranged from 3.62-5.37 per gram in freshly processed samples.
- Bacterial genus diversity was high and maintained throughout frozen storage.
- Pathogen-containing genera were found in significant proportions in the final product.
Conclusions:
- Intervention processing does not significantly reduce overall bacterial load compared to traditional methods.
- The microbial profile, including the presence of pathogens, remains a concern in intervention-processed beef.
- Further investigation is needed to understand the reasons for microbial similarity and ensure safety.
Related Concept Videos
Methods of Controlling Food Spoilage
Food spoilage is caused by microbial growth or by chemical and physical changes, all of which affect the taste, texture, and safety of food.Temperature-Based PreservationRefrigeration at 0–4 °C slows microbial growth and enzyme activity, making it ideal for short-term storage. However, certain spoilage organisms—such as psychrotrophs like Listeria monocytogenes—can still proliferate at these temperatures. Freezing below -18 °C further slows biological processes by forming ice crystals, which...
Pasteurization and Food Preservation
Pasteurization is a widely employed thermal processing technique designed to enhance the safety and shelf life of perishable food and beverages. By subjecting products to specific high temperatures for controlled durations, this method effectively inactivates pathogenic microorganisms and spoilage enzymes without significantly compromising sensory qualities. The technique has been pivotal in food safety management, especially for consumables susceptible to microbial contamination such as milk,...
Principles of Food Preservation
Food spoilage results from microbial growth, enzymatic activity, and environmental factors that gradually degrade the sensory, nutritional, and safety qualities of food. Preservation techniques aim to slow or halt these processes to extend shelf life and maintain product quality.A key concept in food microbiology is the microbial growth curve, which includes four phases: lag, exponential (log), stationary, and death. During the lag phase, bacteria adjust to their environment without significant...
Microbial Spoilage of Food
Microbial food spoilage refers to the degradation of food quality resulting from the metabolic activity of microorganisms such as bacteria, yeasts, and molds. These microbes proliferate on various food substrates depending on factors such as moisture content, nutrient availability, and storage conditions, leading to undesirable sensory and structural changes.Bacteria are primary agents of spoilage in high-moisture, nutrient-dense foods like meat, milk, and vegetables. Microbial spoilage occurs...
Physical Methods for Controlling Microbial Growth: Temperature
Heat is a widely used method to control microbial growth by targeting and denaturing cellular proteins, thereby killing or inactivating microbes. This method's effectiveness is quantified using parameters such as the thermal death point (TDP), thermal death time (TDT), and decimal reduction time (D value). TDP represents the lowest temperature at which all microorganisms in a liquid suspension are eliminated within 10 minutes, whereas TDT is the time necessary to achieve sterilization at a...

