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Updated: May 27, 2026

Measuring Nitrite and Nitrate, Metabolites in the Nitric Oxide Pathway, in Biological Materials using the Chemiluminescence Method
Published on: December 25, 2016
Determination of nitrites in meat systems: An improved procedure
G Binstok1, C A Campos, L N Gerschenson
1Departamento de Industrias, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires-Ciudad Universitaria (1428), Capital Federal, República Argentina.
Abstract:
Various analytical methods for nitrite determination in meat products, based on extraction of the preservative through digestion at 60-80 °C followed by colorimetric analysis gave relatively poor recoveries (44-65%) when applied to raw beef. An improved procedure was developed based on disintegration and dispersion of beef with sand prior to the extraction step and on the use of a higher concentration of N-(1-naphthyl) ethylenediamine dihydrochloride than normally used. The proposed technique resulted in almost 93% recovery of nitrites and showed a high precision revealed in a variation coefficient of 8̃%.
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