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Related Concept Videos

Transducer Mechanism: Nuclear Receptors01:31

Transducer Mechanism: Nuclear Receptors

Nuclear receptors, or NRs, are unique transcription factors that regulate gene transcription and affect the cellular pathways involved in reproduction, development, or metabolism. Their ability to be stimulated by small lipophilic ligands and control vital cellular processes makes them ideal drug targets. Nearly 10-15% of currently prescribed drugs target these receptors.
About 48 different soluble family members of nuclear receptors are identified that can be divided into two main classes:
Nuclear Export01:42

Nuclear Export

The nucleus restricts several proteins within and allows others to pass. The restricted proteins possess a nuclear retention sequence or NRS, anchoring them to the nuclear lamins and preventing their transport to the cytosol. The non-restricted proteins, after their synthesis, are transported to their site of action, such as the cytosol or other organelles, with the help of nuclear export signals or NES.
NES are of three types- the canonical 10-residue long leucine-rich signal and other...
Nuclear Localization Signals and Import01:46

Nuclear Localization Signals and Import

Proteins targeted to the nucleus carry short stretches of amino acid sequences called the nuclear localization signal or NLS. Classical nuclear localization signals are of two types: monopartite and bipartite NLS. Monopartite classical NLS (cNLS) consists of a single cluster of 4-8 amino acids. Bipartite cNLS consists of two clusters of  2-3 amino acids and a 9-12 residue long proline-rich linker bridging the two clusters. Signal clusters are rich in positively charged amino acids such as...
Nuclear Binding Energy02:13

Nuclear Binding Energy

The difference between the calculated and experimentally measured masses is known as the mass defect of the atom. In the case of helium-4, the mass defect indicates a “loss” in mass of 4.0331 amu – 4.0026 amu = 0.0305 amu. The loss in mass accompanying the formation of an atom from protons, neutrons, and electrons is due to the conversion of that mass into energy that is evolved as the atom forms. The nuclear binding energy is the energy produced when the atoms’ nucleons are bound together;...
Regulation of Nuclear Protein Sorting01:45

Regulation of Nuclear Protein Sorting

Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
Signal Transduction: Overview01:26

Signal Transduction: Overview

Cells respond to many types of information, often through receptor proteins positioned on the membrane. They respond to chemical signals, such as hormones, neurotransmitters, and other signaling molecules, initiating a series of molecular reactions to produce an appropriate response. This is called signal transduction. Cells also coordinate different responses elicited by the same signaling molecule via mediators, allowing molecular cross-talk.
Typically, signal transduction involves three...

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Reverse Yeast Two-hybrid System to Identify Mammalian Nuclear Receptor Residues that Interact with Ligands and/or Antagonists
10:51

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Published on: November 15, 2013

NucleaRDB: information system for nuclear receptors.

Bas Vroling1, David Thorne, Philip McDermott

  • 1CMBI, NCMLS, Radboud University Nijmegen Medical Centre, Nijmegen, Bio-Prodict, Dreijenplein 10, 6703 HB Wageningen, The Netherlands.

Nucleic Acids Research
|November 9, 2011
PubMed
Summary

The NucleaRDB is a comprehensive database for nuclear hormone receptors, integrating diverse experimental and computational data. It offers advanced access methods and a unique PDF reader for seamless integration with scientific literature.

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Reverse Yeast Two-hybrid System to Identify Mammalian Nuclear Receptor Residues that Interact with Ligands and/or Antagonists
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Area of Science:

  • Molecular Biology
  • Bioinformatics
  • Pharmacology

Background:

  • Nuclear hormone receptors play crucial roles in various physiological processes.
  • A centralized, validated data resource is needed for nuclear hormone receptor research.
  • Existing data on nuclear hormone receptors are often fragmented and heterogeneous.

Purpose of the Study:

  • To develop and present the NucleaRDB, a Molecular Class-Specific Information System for nuclear hormone receptors.
  • To consolidate diverse experimental and computational data related to nuclear hormone receptors.
  • To provide accessible and integrated information for researchers in the field.

Main Methods:

  • Data collection, combination, and validation from heterogeneous sources.
  • Development of interactive and programmatic query systems for data access.
  • Integration with a nuclear hormone receptor-specific PDF reader for linking to full-text articles.

Main Results:

  • The NucleaRDB successfully integrates large amounts of heterogeneous data on nuclear hormone receptors.
  • Multiple access methods, including interactive and programmatic queries, are available.
  • A specialized PDF reader facilitates the integration of database content with scientific literature.

Conclusions:

  • NucleaRDB serves as a valuable, freely accessible resource for nuclear hormone receptor research.
  • The system enhances data accessibility and integration, supporting further scientific discovery.
  • This comprehensive information system aids researchers in understanding nuclear hormone receptor functions and interactions.