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Updated: May 27, 2026
10:37
Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
Published on: November 15, 2017
Mass spectrometry-based chemoproteomic approaches
1Cellzome AG, Heidelberg, Germany. Bantscheff@cellzome.com
Methods in Molecular Biology (Clifton, N.J.)
|November 9, 2011
Summary
Chemical proteomics investigates how bioactive small molecules affect proteins using advanced techniques. This review covers key experimental approaches, including quantitative mass spectrometry, for understanding drug mechanisms.
Area of Science:
- Integrative research at the intersection of chemistry, biochemistry, and cell biology.
Background:
- Bioactive small molecules are crucial in biological research and drug discovery.
- Understanding their precise mechanism of action requires detailed analysis of protein interactions and cellular responses.
Purpose of the Study:
- To review major experimental methodologies in chemical proteomics.
- To highlight the application of these methods in studying small molecule interactions with the proteome.
Main Methods:
- Focus on quantitative mass spectrometry-based approaches.
- Summarizes diverse experimental types used in chemical proteomics.
Main Results:
- Chemical proteomics enables mapping of small molecule targets.
- It reveals impacts on protein expression and post-translational modifications.
Conclusions:
- Chemical proteomics provides a powerful framework for dissecting small molecule mechanisms of action.
- Quantitative mass spectrometry is a key technology driving advancements in this field.
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