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Related Concept Videos

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

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Related Experiment Video

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Monitoring of Ubiquitin-proteasome Activity in Living Cells Using a Degron (dgn)-destabilized Green Fluorescent Protein (GFP)-based Reporter Protein
10:25

Monitoring of Ubiquitin-proteasome Activity in Living Cells Using a Degron (dgn)-destabilized Green Fluorescent Protein (GFP)-based Reporter Protein

Published on: November 10, 2012

Fluorescence-based proteasome activity profiling.

Annemieke de Jong1, Karianne G Schuurman, Boris Rodenko

  • 1Division of Cell Biology II, The Netherlands Cancer Institute, Amsterdam, The Netherlands.

Methods in Molecular Biology (Clifton, N.J.)
|November 9, 2011
PubMed
Summary
This summary is machine-generated.

A new fluorescent probe enables sensitive detection of proteasome activity in various biological samples. This tool aids in monitoring proteasome inhibitor efficacy for cancer therapy.

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Last Updated: May 27, 2026

Monitoring of Ubiquitin-proteasome Activity in Living Cells Using a Degron (dgn)-destabilized Green Fluorescent Protein (GFP)-based Reporter Protein
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Use of the Protease Fluorescent Detection Kit to Determine Protease Activity
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Detection of Protease Activity by Fluorescent Peptide Zymography
09:56

Detection of Protease Activity by Fluorescent Peptide Zymography

Published on: January 20, 2019

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Cancer Therapeutics

Background:

  • The proteasome is a key therapeutic target in cancer treatment.
  • Accurate monitoring of proteasome activity and inhibitor efficacy is crucial.
  • Existing methods for proteasome activity profiling can be labor-intensive.

Purpose of the Study:

  • To develop and validate a novel fluorescent probe for proteasome activity.
  • To enable sensitive and direct profiling of proteasomal activity.
  • To provide a versatile tool for various biological sample types and experimental setups.

Main Methods:

  • Synthesis of a novel fluorescent proteasome activity probe.
  • Application of the probe for activity profiling in cell lysates, intact cells, and patient-derived materials.
  • Analysis using SDS-PAGE, confocal laser scanning microscopy, and flow cytometry.

Main Results:

  • The fluorescent probe allows for high-sensitivity detection of proteasomal activity.
  • Direct scanning of SDS-PAGE gels for fluorescent emission of proteasomal subunits is feasible, bypassing Western blot analysis.
  • The probe demonstrates suitability for microscopy and flow cytometry applications.

Conclusions:

  • The developed fluorescent probe is a sensitive and versatile tool for monitoring proteasome activity.
  • This probe facilitates accurate profiling of proteasome (inhibitor) activity in diverse biological contexts.
  • It offers a streamlined alternative to conventional methods for proteasome activity assessment in cancer research.