Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Segmental copy number amplifications are more stable than aneuploidies in the absence of selection.

Molecular biology and evolution·2026
Same author

Investigating the distribution of antibiotic resistance genes in relation to bacterial, fungal, and functional diversity in a hay field.

Microbiology spectrum·2026
Same author

Quiescence improves <i>Candida albicans</i> survival of fungicidal drug exposure.

bioRxiv : the preprint server for biology·2026
Same author

Intracellular replication dynamics of influenza A virus impose strong bottleneck effects.

bioRxiv : the preprint server for biology·2025
Same author

Copy number variation facilitates rapid toggling between ecological strategies.

bioRxiv : the preprint server for biology·2025
Same author

Segmental copy number amplifications are stable in the absence of selection.

bioRxiv : the preprint server for biology·2025
Same journal

Mapping the 3D Chromosome Organization of a Biosynthetic Gene Cluster by Capture Hi-C (CHi-C).

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Mapping the 3D Chromosome Organization of Streptomyces by Hi-C.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

CUT&Tag Epigenomic Profiling of Biosynthetic Gene Clusters in Arabidopsis thaliana.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Rhizobium rhizogenes-Mediated Hairy Root Transformation Protocol for Lotus japonicus and Other Legumes.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Characterization of Bioactive Saponins from Sea Cucumbers.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Methods for Functional Validation of Terpenoid Metabolic Clusters in Nicotiana benthamiana and Aspergillus oryzae.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Related Experiment Video

Updated: May 27, 2026

An Array-based Comparative Genomic Hybridization Platform for Efficient Detection of Copy Number Variations in Fast Neutron-induced Medicago truncatula Mutants
09:32

An Array-based Comparative Genomic Hybridization Platform for Efficient Detection of Copy Number Variations in Fast Neutron-induced Medicago truncatula Mutants

Published on: November 8, 2017

DNA microarray-based mutation discovery and genotyping.

David Gresham1

  • 1Department of Biology and Center for Genomics and Systems Biology, New York University, New York, NY, USA. dgresham@nyu.edu

Methods in Molecular Biology (Clifton, N.J.)
|November 9, 2011
PubMed
Summary
This summary is machine-generated.

DNA microarrays efficiently identify single-nucleotide polymorphisms (SNPs). Probe melting temperature (Tm) is key to sensitivity, enabling precise SNP discovery and genotyping in mixed DNA samples.

More Related Videos

Infinium Assay for Large-scale SNP Genotyping Applications
13:33

Infinium Assay for Large-scale SNP Genotyping Applications

Published on: November 19, 2013

Wild-type Blocking PCR Combined with Direct Sequencing as a Highly Sensitive Method for Detection of Low-Frequency Somatic Mutations
10:41

Wild-type Blocking PCR Combined with Direct Sequencing as a Highly Sensitive Method for Detection of Low-Frequency Somatic Mutations

Published on: March 29, 2017

Related Experiment Videos

Last Updated: May 27, 2026

An Array-based Comparative Genomic Hybridization Platform for Efficient Detection of Copy Number Variations in Fast Neutron-induced Medicago truncatula Mutants
09:32

An Array-based Comparative Genomic Hybridization Platform for Efficient Detection of Copy Number Variations in Fast Neutron-induced Medicago truncatula Mutants

Published on: November 8, 2017

Infinium Assay for Large-scale SNP Genotyping Applications
13:33

Infinium Assay for Large-scale SNP Genotyping Applications

Published on: November 19, 2013

Wild-type Blocking PCR Combined with Direct Sequencing as a Highly Sensitive Method for Detection of Low-Frequency Somatic Mutations
10:41

Wild-type Blocking PCR Combined with Direct Sequencing as a Highly Sensitive Method for Detection of Low-Frequency Somatic Mutations

Published on: March 29, 2017

Area of Science:

  • Molecular Biology
  • Genetics
  • Bioinformatics

Background:

  • DNA microarrays are crucial tools for identifying single-nucleotide polymorphisms (SNPs) and determining their frequencies in various DNA samples.
  • Understanding the factors influencing probe sensitivity is essential for optimizing SNP detection accuracy.

Purpose of the Study:

  • To investigate parameters affecting DNA probe sensitivity to SNPs.
  • To develop an isothermal-melting temperature DNA microarray design for enhanced SNP analysis.
  • To provide guidelines for designing and implementing such microarrays for SNP discovery, genotyping, and allele frequency determination.

Main Methods:

  • Studied the relationship between probe melting temperature (Tm) and SNP detection sensitivity.
  • Designed an isothermal-melting temperature DNA microarray by adjusting probe lengths.
  • Developed protocols for DNA sample labeling and hybridization to the microarrays.

Main Results:

  • Identified probe melting temperature (Tm) as the primary determinant of DNA probe sensitivity to SNPs.
  • Demonstrated that an isothermal-melting temperature design, achieved by varying probe lengths, tightly distributes Tm values.
  • Validated the utility of this microarray design for SNP discovery, genotyping, and quantifying allele frequencies in mixed samples.

Conclusions:

  • Probe Tm is a critical factor for sensitive SNP identification using DNA microarrays.
  • Isothermal-melting temperature DNA microarray design offers a robust platform for accurate SNP analysis.
  • The developed guidelines and protocols facilitate efficient SNP discovery and frequency determination in diverse sample types.