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Application of long synthetic oligonucleotides for gene analysis: effect of probe length and stringency conditions on
Y Kajimura1, J Krull, S Miyakoshi
1Department of Molecular Genetics, Beckman Research Institute of the City of Hope, Duarte, CA.
Genetic Analysis, Techniques and Applications
|June 1, 1990
Abstract:
Two different lengths of long unique synthetic oligonucleotide probes (37- and 48-mers) specific for human major histocompatibility complex (MHC) class II beta genes were synthesized. These oligonucleotides were utilized to examine factors influencing hybridization specificity. Both probe length and stringency of washing conditions were found to be crucial factors for sequence-specific hybridization.