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Electropolymerized poly(Toluidine blue)-modified carbon felt for highly sensitive amperometric determination of NADH
Yasushi Hasebe1, Yue Wang, Kazuya Fukuoka
1Department of Life Science and Green Chemistry, Saitama Institute of Technology, 1690 Fusaiji, Fukaya, Saitama 369-0293, Japan. hasebe@sit.ac.jp
Abstract:
Poly(pheniothiazine) films were prepared on a porous carbon felt (CF) electrode surface by an electrooxidative polymerization of three phenothiazine derivatives (i.e.,Tthionine (TN), Toluidine Blue (TB) and Methylene Blue (MB)) from 0.1 mol/L phosphate buffer solution (pH 7.0). Among the three phenothiazies, the poly(TB) film-modified CF exhibited an excellent electrocatalytic activity for the oxidation of nicotinamide adenine dinucleotide reduced form (NADH) at +0.2 V vs. Ag/AgCl. The poly(TB) film-modified CF was successfully used as working electrode unit of highly sensitive amperometric flow-through detector for NADH. The peak currents (peak heights) were almost unchanged, irrespective of a carrier flow rate ranging from 2.0 to 4.1 mL/min, resulting in the measurement of NADH (ca. 30 samples/hr) at 4.1 mL/min. The peak current responses of NADH showed linear relationship over the concentration range from 1 to 30 micromol/L (sensitivity: 0.318 microA/(micromol/L); correlation coefficient: 0.997). The lower detection limit was found to be 0.3 micromol/L (S/N = 3).
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