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[Fingerprints of Rosae Rugosae Flos by HPLC]
Mahinur Bakri1, Jun Chen, Jie Zhong
1Xinjiang Key Laboratory of Plant Resources and Natural Products Chemistry, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi 830011, China. mahinur@ms.xjb.ac.cn
Objective:
To establish a HPLC fingerprint of Rosae Rugosae Flos.
Methods:
Inertsil ODS-3 (4.6 mm x 250 mm, 5 microm) was used with the mixture of methanol, acetonitrile and 0.06% TFA as mobile phase. The flow rate was 1.0 mL/min, the column temperature was 32degrees C, and the detection wavelength was 254 nm.
Results:
HPLC fingerprint of 9 main peaks was established preliminarily from 13 samples of Rosae Rugosae Flos which achieved the technical requirements of the fingerprint of traditional Chinese medicine.
Conclusion:
The method is reliable, accurate and can be used for quality comtrol of Rosae Rugosae Flos.
