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Updated: May 27, 2026

Field Collection and Laboratory Routine Identification of Rhodiola crenulata
Published on: October 27, 2023
[Study on preparation of salidroside and polysaccharide in Rhodiola crenulata]
Cong-qing Wang1, Juan Chen, Cai-dong Li
1Lanzhou Institute of Liver Disease, the No. 2 People's Hospital of Lanzhou, Lanzhou 730046, China. wcq.666@163.com
Objective:
To optimize the preparation process of salidroside and polysaccharide in Rhodiola crenulata.
Methods:
Water-extraction and alcohol-precipitation was used. The effect of added water volume, decoction time and frequency on water-extracting process was investigated by L9 (3(4)) orthogonal design using extraction rate, content of salidroside and polysaccharide as the assessment indexes. The effect of liquor strength and ethanol concentration on alcohol-precipitating process was investigated as well, using content of salidroside and polysaccharide as the assessment indexes.
Results:
The optimum conditions for water-extracting process were as follows: decocting 3 times, and each time adding 8 fold of water and extracting 2 h. For alcohol-precipitating process,it was that liquor strength was 0.4 g/mL, and ethanol concentration in liquor was 70%.
Conclusion:
The optimized process has guidance for the development and comprehensive utilization of this plant.
