Related Experiment Video
Updated: May 27, 2026

Murine Excisional Wound Healing Model and Histological Morphometric Wound Analysis
Published on: August 21, 2020
Quantitative estimation of exudate volume for full-thickness pressure ulcers: the ESTimation method
S Iizaka1, H Sanada, G Nakagami
1Department of Gerontological Nursing/Wound Care Management, Division Health Sciences and Nursing, Graduate School of Medicine, The University of Tokyo, Japan. iizaka-tky@umin.ac.jp
Objective:
To develop an equation for the estimation of exudate volume in millilitres, for full-thickness pressure ulcers, according to wound characteristics.
Method:
In a cross-sectional study, 41 patients aged >60 years with 58 full-thickness pressure ulcers were evaluated. Exudate was collected by covering each wound with a transparent occlusive dressing and the accumulated volume was measured to estimate volume per day. The overall severity of each wound was evaluated by the DESIGN-R tool; a model was then developed to estimate the volume of exudate based on these experimental values. Linear regression analyses were performed to evaluate the precision and accuracy of the model.
Results:
The model, including exudate score, size score, and total score, showed a higher adjusted coefficient of determination (R²=0.66) than the model with only a traditional exudate score (R²=0.57). After adjustment for age, inclusion of interaction terms, and modification of bias, a model with continuous parameters was finally developed: exudate volume per day (ml/day) = exp([0.86×exudate score]+ [0.21×size score]+[0.12×total score]-[0.013×size score×total score]-[0.04×age]-3.60). Furthermore, a categorical model was developed for clinical simplicity of use. The adjusted R2 was increased to 0.73 for the continuous model and to 0.77 for the categorical model. There were no apparent biases (p>0.05) and no correlations between residuals and measured value (p>0.05) in these models.
Conclusion:
The equation, including the exudate score, size score and total score of DESIGN-R, as well as age, is called the ESTimation method. It will be useful for clinicians to predict the absolute volume of exudate and to select appropriate dressings for full-thickness pressure ulcers.
Declaration Of Interest:
The authors have nothing to declare.
Related Concept Videos
Area Problem
One-Compartment Open Model: Urinary Excretion Data and Determination of k
Calculation of Volume of Solids by Integration
Estimation of the Physical Quantities
Inflammatory Response II: Inflammatory Exudate and Tissue Repair
The typical wound exudate is odorless, transparent, straw-colored, thin, and watery. Exudate, however, can differ depending on the state of wound healing. Likewise, the exudate's...
One-Compartment Open Model: Wagner-Nelson and Loo Riegelman Method for ka Estimation
On...

