Related Experiment Video
Updated: May 27, 2026
06:31
"Cell Surface Capture" Workflow for Label-Free Quantification of the Cell Surface Proteome
Published on: March 24, 2023
The quantitative proteome of a human cell line
Martin Beck1, Alexander Schmidt, Johan Malmstroem
1European Molecular Biology Laboratory, Heidelberg, Germany.
Molecular Systems Biology
|November 10, 2011
Summary
This study quantifies human cell proteomes in interphase and mitosis, revealing over 10,000 proteins with concentrations varying seven orders of magnitude. Understanding protein abundance is crucial for systems biology and evolutionary insights.
Area of Science:
- Systems Biology
- Proteomics
- Cell Biology
Background:
- Mathematical modeling and simulations in systems biology require absolute quantities of cellular components.
- Systematic estimates of cellular protein concentrations are scarce, limiting quantitative biological studies.
Purpose of the Study:
- To provide a quantitative description of the human cell proteome in two distinct functional states: interphase and mitosis.
- To establish a comprehensive dataset of protein concentrations for a commonly used human cell line.
Main Methods:
- Quantitative proteomic analysis of a human cell line.
- Measurement of protein concentrations across a wide dynamic range.
Main Results:
- Human cultured cells express at least 10,000 proteins.
- Quantified proteins exhibit concentrations spanning seven orders of magnitude, up to 20 million copies per cell.
- Differences in proteome composition between interphase and mitosis were observed.
Conclusions:
- This work provides essential quantitative proteomic data for systems biology research.
- The findings offer insights into the relationship between protein abundance, cellular function, and evolution.
- The study highlights the vast dynamic range of protein concentrations within human cells.
