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Updated: May 27, 2026
Generation of Monoclonal Antibodies Against Natural Products
Published on: April 6, 2019
Development and characterization of a monoclonal antibody against Ochratoxin B and its application in ELISA
Alexandra H Heussner1, Simon Ausländer, Daniel R Dietrich
1Human and Environmental Toxicology, University of Konstanz, PO-Box 918, 78457 Konstanz, Germany. alexandra.heussner@uni-konstanz.de
Abstract:
A monoclonal antibody specific to ochratoxin B (OTB) was employed for the development of an indirect competitive OTB-ELISA. The optimized OTB-ELISA resulted in a limit of detection (LOD) for OTB of 3 µg/L (8 nM), a limit of quantification (LOQ) of 3.7 µg/L (10 nM), and a 50% inhibitory concentration (IC(50)) of 150 nM. Due to very low cross-reactivity to OTA (2.7%) and structurally related molecules (0%), this OTB-ELISA was found to be suitable to detect OTB with excellent precision in different matrices, i.e., beer, coffee and wine. Therefore, this OTB-ELISA will allow screening of OTB in food and feed products.
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