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Related Concept Videos

High-Performance Liquid Chromatography: Introduction01:11

High-Performance Liquid Chromatography: Introduction

High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process:
High-Performance Liquid Chromatography: Elution Process01:05

High-Performance Liquid Chromatography: Elution Process

In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...
Ion-Exchange Chromatography01:09

Ion-Exchange Chromatography

Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...
High-Performance Liquid Chromatography: Instrumentation00:57

High-Performance Liquid Chromatography: Instrumentation

High-performance liquid chromatography, or HPLC, is an analytical technique that separates liquid samples under high pressures. An HPLC instrument consists of glass bottles for storing solvents called mobile phase reservoirs. HPLC-grade solvents are used to maintain high purity, and the dissolved gases are removed using a degasser, such as a vacuum pumping system or sparging with helium. The solvents are then pumped into the analytical column using a screw-driven syringe or reciprocating pumps.
Chromatography: Introduction01:10

Chromatography: Introduction

Chromatography is a technique used to separate compounds based on differences of partitioning between two phases, the stationary phase and the mobile phase.
The phase in which the compounds linger or on which the compounds adsorb is called the stationary phase, whereas the mobile phase is the solvent that carries the solutes to be analyzed. In traditional column chromatography, the mixture flows through the stationary phase, and the compounds partition between the stationary and mobile phases...
Size-Exclusion Chromatography01:08

Size-Exclusion Chromatography

In size-exclusion chromatography (SEC), also known as molecular-exclusion or gel-permeation chromatography, molecules are separated based on their sizes. This technique is important for separating large molecules such as polymers and biomolecules. The two classes of micron-sized stationary phases encountered in SEC are silica particles and cross-linked polymer resin beads. Both materials are porous, but their pore sizes vary significantly.
Silica particles offer advantages such as rigidity,...

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Fabrication of a Dipole-assisted Solid Phase Extraction Microchip for Trace Metal Analysis in Water Samples
09:42

Fabrication of a Dipole-assisted Solid Phase Extraction Microchip for Trace Metal Analysis in Water Samples

Published on: August 7, 2016

Liquid phase chromatography on microchips.

Jörg P Kutter1

  • 1Department of Micro and Nanotechnology, Technical University of Denmark, Ørsteds Plads, 2800 Kongens Lyngby, Denmark. joerg.kutter@nanotech.dtu.dk

Journal of Chromatography. A
|November 11, 2011
PubMed
Summary
This summary is machine-generated.

Microfluidics enables miniaturized chemical analysis systems like Lab-on-a-Chip devices. This review critically examines liquid chromatography on microchips, highlighting key considerations and remaining challenges in miniaturized separations.

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Area of Science:

  • Analytical Chemistry
  • Microfluidics Engineering

Background:

  • Microfluidics has enabled miniaturized chemical analysis systems, known as Lab-on-a-Chip (LOC) systems.
  • Initial interest from analytical chemistry focused on miniaturized sensors, detectors, and separation systems.
  • Chromatographic separation systems on microchips saw rapid early development, which has since slowed.

Purpose of the Study:

  • To critically review the implementation of liquid phase chromatography in miniaturized formats.
  • To identify critical factors for developing and utilizing miniaturized separation systems.
  • To outline persistent challenges and potential pitfalls in microchip chromatography.

Main Methods:

  • Literature review of microfluidic chromatographic systems.
  • Analysis of engineering-driven advancements in microfluidics.
  • Critical assessment of analytical chemistry applications in miniaturized separations.

Main Results:

  • Microfluidics is a key technology for Lab-on-a-Chip (LOC) systems.
  • Early development of microchip chromatography has slowed.
  • This review critically examines miniaturized liquid phase chromatography.

Conclusions:

  • Microfluidics offers significant potential for miniaturized analytical systems.
  • Further research is needed to address challenges in microchip chromatography.
  • Understanding limitations is crucial for advancing miniaturized separation technologies.