Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Third position base changes in codons 5' and 3' adjacent UGA codons affect UGA suppression in vivo.

R H Buckingham1, P Sörensen, F T Pagel

  • 1Institut de Biologie Physico-Chimique, Paris, France.

Biochimica Et Biophysica Acta
|August 27, 1990
PubMed
Summary

Nonsense codon context influences suppression efficiency. Upstream codons and downstream wobble bases significantly impact this process, affecting suppressor tRNA activity and termination.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

First Limits on Light Dark Matter Interactions in a Low Threshold Two-Channel Athermal Phonon Detector from the TESSERACT Collaboration.

Physical review letters·2025
Same author

First Constraint on Atmospheric Millicharged Particles with the LUX-ZEPLIN Experiment.

Physical review letters·2025
Same author

New Constraints on Cosmic Ray-Boosted Dark Matter from the LUX-ZEPLIN Experiment.

Physical review letters·2025
Same author

Dark Matter Search Results from 4.2  Tonne-Years of Exposure of the LUX-ZEPLIN (LZ) Experiment.

Physical review letters·2025
Same author

Erratum: Measurement of the Sixth-Order Cumulant of Net-Proton Multiplicity Distributions in Au+Au Collisions at sqrt[s_{NN}]=27, 54.4, and 200 GeV at RHIC [Phys. Rev. Lett. 127, 262301 (2021)].

Physical review letters·2025
Same author

Erratum: Nonmonotonic Energy Dependence of Net-Proton Number Fluctuations [Phys. Rev. Lett. 126, 092301 (2021)].

Physical review letters·2025

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • Nonsense codons (stop signals) in mRNA can lead to premature protein termination.
  • The sequence context surrounding nonsense codons is known to influence the efficiency of nonsense-mediated decay and suppression.
  • Previous studies highlighted the importance of bases downstream of the nonsense codon.

Purpose of the Study:

  • To investigate the impact of upstream and downstream codon sequences on nonsense codon suppression efficiency.
  • To determine if the wobble base of the downstream codon affects suppression.
  • To elucidate the mechanisms by which sequence context influences suppressor tRNA activity.

Main Methods:

  • Site-directed mutagenesis was used to alter serine codons flanking a nonsense mutation (UGA234) in the Escherichia coli trpA gene.

Related Experiment Videos

  • The efficiency of nonsense suppression was assessed using a UGA nonsense suppressor derived from glyT.
  • Mutant strains with modified codon contexts were analyzed for protein expression levels.

    • Main Results:

    • The sequence of the upstream codon significantly affects nonsense suppression efficiency.
    • The third (wobble) base of the downstream codon also influences suppression.
    • These findings expand upon previous observations implicating only downstream bases.

    Conclusions:

    • Both upstream and downstream codon sequences play critical roles in modulating nonsense codon suppression.
    • The effects of downstream bases may involve termination processes, while upstream and wobble base effects might directly impact suppressor tRNA function.
    • Understanding codon context is crucial for optimizing gene expression and studying translation termination and suppression mechanisms.