[Accumulation of UV-induced radicals in proteins with various degree of aggregation]

Accumulation of radicals UV-induced in protein solutions at 77 K was investigated. It has been found that in each molecule of small proteins true solutions only one ionization proceeds and two radicals are formed. The limiting number of radicals per one protein molecule decreases with the increase of their aggregation level. A method is suggested for observing aggregation and desegregation processes in proteins, as well as a method for determining the absolute concentration of radicals.