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Updated: May 27, 2026

In Situ Hybridization Techniques for Paraffin-Embedded Adult Coral Samples
Published on: August 31, 2018
Bioactive cembranoids from the soft coral Sinularia crassa
Chih-Hua Chao1,2, Kuei-Ju Chou1, Chiung-Yao Huang1
1Department of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung 804, Taiwan.
Abstract:
Eight new cembranoids, crassarines A-H (1-8) were isolated from the Formosan soft coral Sinularia crassa. Compounds 1-3 represent the rare cembranoids with a 1,12-oxa-bridged tetrahydrofuran ring, while 4 and 5 are the firstly discovered 1,11-oxa-bridged tetrahydropyranocembranoids. The absolute configuration of 6 was determined using the Mosher's method. Compounds 6 and 8 were found to significantly inhibit the expression of both pro-inflammatory iNOS and COX-2 proteins at 10 μM, respectively, while compounds 4-8 were found to be non-cytotoxic toward the selected human liver cancer cells.
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