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Related Concept Videos

Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
Two-Dimensional Microscopy in Microbiology01:29

Two-Dimensional Microscopy in Microbiology

Two-dimensional (2D) microscopy encompasses a range of optical techniques that capture images within a single focal plane, offering detailed representations of microscopic structures. These techniques are essential in biological and medical research, enabling the visualization of cellular and subcellular structures with different levels of contrast and specificity.There are several major types of 2D microscopy, each with strengths and applications.Bright-Field MicroscopyBright-field microscopy...
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...

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Related Experiment Video

Updated: May 27, 2026

Corneal Confocal Microscopy: A Novel Non-invasive Technique to Quantify Small Fibre Pathology in Peripheral Neuropathies
11:29

Corneal Confocal Microscopy: A Novel Non-invasive Technique to Quantify Small Fibre Pathology in Peripheral Neuropathies

Published on: January 3, 2011

Micrometer axial resolution OCT for corneal imaging.

Rahul Yadav, Kye-Sung Lee, Jannick P Rolland

    Biomedical Optics Express
    |November 15, 2011
    PubMed
    Summary
    This summary is machine-generated.

    A new optical coherence tomography (OCT) system provides high-resolution corneal imaging using a supercontinuum light source. This advanced OCT technology accurately quantifies thin corneal layers in various eye conditions.

    Keywords:
    (170.0110) Imaging systems(170.4460) Ophthalmic optics and devices(170.4500) Optical coherence tomography

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    Corneal Confocal Microscopy: A Novel Non-invasive Technique to Quantify Small Fibre Pathology in Peripheral Neuropathies
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    Published on: January 3, 2011

    A Custom Multiphoton Microscopy Platform for Live Imaging of Mouse Cornea and Conjunctiva
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    In vivo Structural Assessments of Ocular Disease in Rodent Models using Optical Coherence Tomography
    07:44

    In vivo Structural Assessments of Ocular Disease in Rodent Models using Optical Coherence Tomography

    Published on: July 24, 2020

    Area of Science:

    • Ophthalmology
    • Biomedical Optics
    • Medical Imaging

    Background:

    • Accurate corneal imaging is crucial for diagnosing and monitoring eye diseases.
    • Existing optical coherence tomography (OCT) systems may face limitations in resolution and dispersion management for detailed corneal layer analysis.

    Purpose of the Study:

    • To develop and present a novel optical coherence tomography (OCT) system for high axial resolution corneal imaging.
    • To evaluate the system's capability in quantifying thin corneal structures in vivo.

    Main Methods:

    • Utilized a broadband supercontinuum light source with a 375 nm bandwidth (625–1000 nm).
    • Designed the system in free space to minimize dispersion and implemented a custom Czerny-Turner spectrometer.
    • Achieved an imaging depth of 1 mm with an experimentally measured axial resolution of 1.1 μm in corneal tissue.

    Main Results:

    • Demonstrated high axial resolution (1.1 μm) in corneal tissue, consistent with theoretical calculations.
    • Successfully performed in vivo imaging, quantifying thin corneal layers like the tear film and Bowman's layer.
    • Showcased the system's ability to differentiate between normal, keratoconus, and contact lens-wearing eyes.

    Conclusions:

    • The developed OCT system offers high-resolution corneal imaging capabilities.
    • The system is suitable for various ophthalmic applications, including the analysis of corneal pathologies and contact lens interactions.
    • Quantification of thin corneal layers is feasible with this advanced OCT technology.