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Updated: May 27, 2026

An Improved and High Throughput Respiratory Syncytial Virus (RSV) Micro-neutralization Assay
Published on: January 26, 2019
A respiratory syncytial virus isolate enables the testing of virucidal products
Thomas Thevenin1, P E Lobert, A Dewilde
1Laboratory of Virology/EA3610, University Lille 2, Faculty of Medecine, CHRU Lille, 59037 Lille, France.
Abstract:
The respiratory syncytial virus (RSV) is known as a major cause of respiratory infections and nosocomial diseases. Testing this virus is rather difficult due to the problems encountered in producing it at a high titer without using any purification method. A RSV isolate which replicates to high level on a Hep-2 cell line with an infectious titer of at least 10(7)TCID(50)mL(-1) in culture supernatant fluids has been identified. Thanks to this isolate, the virucidal effects of two products, a hand rub solution and a surface disinfectant, were conveniently tested according to the EN 14476:2007-02 procedure.

