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Updated: May 27, 2026

Identification of Mouse and Human Antibody Repertoires by Next-Generation Sequencing
Published on: March 15, 2019
A database of immunoglobulins with integrated tools: DIGIT
Anna Chailyan1, Anna Tramontano, Paolo Marcatili
1Department of Physics, Sapienza University of Rome, P.le A Moro 5-00185, Italy.
The DIGIT database offers annotated immunoglobulin variable domain sequences with integrated analysis tools. It provides antigen, germline, and pairing information, aiding antibody research.
Area of Science:
- Immunology
- Bioinformatics
- Structural Biology
Background:
- Immunoglobulin variable domains are crucial for antibody diversity and antigen recognition.
- Analyzing these domains requires integrated resources for sequence and structural data.
- Existing databases may lack comprehensive annotation and analysis tools.
Purpose of the Study:
- To present the DIGIT (Database of ImmunoGlobulins with Integrated Tools) database.
- To provide a comprehensive resource for annotated immunoglobulin variable domain sequences.
- To offer integrated tools for searching and analyzing immunoglobulin data.
Main Methods:
- Database development integrating sequence and annotation data.
- On-the-fly computation of annotations like CDR identification and germline mutations.
- Implementation of tools for sequence searching (BLAST) and 3D model building.
Main Results:
- DIGIT stores annotated immunoglobulin variable domain sequences.
- Annotations include antigen type, germline sequences, and chain pairing information.
- On-the-fly annotations and analysis tools are available for user-submitted sequences.
Conclusions:
- DIGIT serves as a valuable integrated resource for immunoglobulin research.
- The database facilitates in-depth analysis of antibody variable domains.
- It supports customized searches and structural modeling for antibody discovery.
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