Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Disorders of Erythrocytes

Disorders of Erythrocytes

Disorders of erythrocytes, or red blood cells (RBCs), include a range of conditions affecting their number, shape, or function.
Erythrocyte disorders can be broadly categorized into two main types: anemic and polycythemic conditions.
A low oxygen-carrying capacity of the blood due to the loss, lower production, or destruction of erythrocytes is termed anemia. Hemorrhagic anemia, for example, occurs when bleeding from an external wound or internal ulcer reduces erythrocyte counts.
On the other...

Hemoglobin

Hemoglobin

Hemoglobin is a globular protein made up of four subunits. Two of these subunits are alpha chains, and the other two are beta chains. Each subunit contains a molecule of heme, which has an iron atom and can bind to oxygen. When an oxygen molecule binds to one heme group, it changes the shape of hemoglobin, making it easier for the other heme groups to bind oxygen as well.
When all four heme groups are bound to oxygen, the resulting molecule is called oxyhemoglobin. As a result, arterial blood...

Multiple Allele Traits

Multiple Allele Traits

The Concept of Multiple Allelism

Oxygen Transport in the Blood

Oxygen Transport in the Blood

Hemoglobin (Hb) is a crucial molecule in the human body, consisting of four polypeptide chains, each bound to an iron-containing heme group. This unique structure enables hemoglobin to bind to oxygen, with each molecule capable of combining with four molecules of oxygen, leading to rapid and reversible oxygen loading. When fully loaded with oxygen, it is called oxyhemoglobin, while hemoglobin that has released oxygen is called reduced hemoglobin or deoxyhemoglobin. As hemoglobin binds oxygen,...

Blood Types

Blood Types

Human blood is classified into different types based on the presence of antigens on the red blood cell's surface and antibodies in the plasma. Proper identification of blood type is essential for successful blood transfusion. The International Society of Blood Transfusion has identified 38 human blood types based on the surface antigens on the red blood cells. The most common types are ABO, Rh, and MNS blood types.
ABO blood group
ABO antigens are glycoproteins encoded by genes present on...

Rh Blood Group

Rh Blood Group

The Rhesus (Rh) antigen is crucial in determining blood groups and ensuring compatibility during blood transfusions.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Original Article: Evaluation of eosinopenia as a diagnostic and prognostic indicator in COVID-19 infection.

International journal of laboratory hematology·2021

Same author

Significance of RDW in predicting mortality in COVID-19-An analysis of 622 cases.

International journal of laboratory hematology·2021

Same author

D-dimer level is a useful predictor for mortality in patients with COVID-19: Analysis of 483 cases.

Diabetes & metabolic syndrome·2021

Same author

Urine abnormalities predict acute kidney injury in COVID-19 patients: An analysis of 110 cases in Chennai, South India.

Diabetes & metabolic syndrome·2020

Same author

Evaluation of eosinopenia as a diagnostic and prognostic indicator in COVID-19 infection.

International journal of laboratory hematology·2020

Same author

How Reliable Is Automated Urinalysis in Acute Kidney Injury?

Laboratory medicine·2020

Search research articles

Related Experiment Video

Updated: May 27, 2026

Continuous Manual Exchange Transfusion for Patients with Sickle Cell Disease: An Efficient Method to Avoid Iron Overload

Continuous Manual Exchange Transfusion for Patients with Sickle Cell Disease: An Efficient Method to Avoid Iron Overload

Published on: March 14, 2017

Hemoglobin disorders in South India.

Vani Chandrashekar¹, Mamta Soni

¹Department of Hematology, Apollo Hospitals, 21, Greams Lane, Off Greams Road, Chennai 600006, India.

ISRN Hematology

|November 16, 2011

Summary

Cation exchange-high performance liquid chromatography (CE-HPLC) effectively identifies hemoglobinopathies like beta-thalassemia trait and HbE trait. This blood test aids in diagnosing various hemoglobin disorders, including sickle cell disease and thalassemia major.

Area of Science:

Hematology
Clinical Chemistry

Background:

Hemoglobinopathies and thalassemias are significant global health concerns.
Accurate diagnosis is crucial for effective management and genetic counseling.

Purpose of the Study:

To evaluate the utility of cation exchange-high performance liquid chromatography (CE-HPLC) in diagnosing hemoglobinopathies.
To determine the prevalence of different hemoglobin disorders in a South Indian population.

Main Methods:

A one-year retrospective study was conducted at Apollo Hospitals, Chennai.
543 abnormal chromatogram patterns were analyzed using CE-HPLC.
Results were correlated with complete blood count data.

Main Results:

The most common disorders identified were beta-thalassemia trait (37.9%) and HbE trait (23.2%).

More Related Videos

Detection of Residual Donor Erythroid Progenitor Cells after Hematopoietic Stem Cell Transplantation for Patients with Hemoglobinopathies

Detection of Residual Donor Erythroid Progenitor Cells after Hematopoietic Stem Cell Transplantation for Patients with Hemoglobinopathies

Published on: September 6, 2017

Related Experiment Videos

Last Updated: May 27, 2026

Continuous Manual Exchange Transfusion for Patients with Sickle Cell Disease: An Efficient Method to Avoid Iron Overload

Continuous Manual Exchange Transfusion for Patients with Sickle Cell Disease: An Efficient Method to Avoid Iron Overload

Published on: March 14, 2017

Detection of Residual Donor Erythroid Progenitor Cells after Hematopoietic Stem Cell Transplantation for Patients with Hemoglobinopathies

Detection of Residual Donor Erythroid Progenitor Cells after Hematopoietic Stem Cell Transplantation for Patients with Hemoglobinopathies

Published on: September 6, 2017

Other prevalent conditions included homozygous HbE disease (18.9%) and HbS trait (5.3%).

Specific average values for HbA2, HbF, HbE, and HbS were recorded for various conditions.

Conclusions:

CE-HPLC is a valuable tool for the initial investigation of hemoglobinopathies and thalassemias.
The study highlights the epidemiological profile of hemoglobin disorders in the studied region.
Combined with CBC, CE-HPLC effectively categorizes hemoglobinopathies into traits, homozygous, and compound heterozygous states.