Improved method for identifying alpha 1-antitrypsin Pi M subtypes by isoelectric focusing in agarose
1Department of Laboratory Medicine and Pathology, University of Minnesota Hospital and Clinic, Minneapolis 55455.
Abstract:
We describe an improved method for the classification of alpha 1-antitrypsin variants by isoelectric focusing in agarose. Identification of the three Pi M subtypes can now be made by using a narrow-range carrier ampholyte (pH 4.2-4.9) and pretreating serum with dithioerythritol-iodoacetic acid to enhance band resolution. Phenotype results for two groups of Pi M homo- and heterozygotes are compared to illustrate the improved accuracy of the new method.
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