Related Experiment Videos
Preventing ascorbate interference in ion-chromatographic determinations of urinary oxalate: four methods compared.
M Petrarulo1, M Marangella, O Bianco
1Laboratorio Calcolosi Renale, Ospedale Mauriziano Umberto I, Torino, Italy.
Clinical Chemistry
|September 1, 1990
Summary
To improve urinary oxalate measurement, four methods were tested to remove interfering ascorbate. Boric acid dilution is effective for routine use, while ascorbate oxidase is a reliable alternative.
Area of Science:
- Analytical Chemistry
- Biochemistry
Background:
- Ascorbate (vitamin C) interferes with ion-chromatographic determination of urinary oxalate.
- Accurate urinary oxalate measurement is crucial for diagnosing conditions like kidney stones.
Purpose of the Study:
- To compare four methods for eliminating ascorbate interference in urinary oxalate analysis.
- To identify effective and practical methods for routine clinical use.
Main Methods:
- Tested four ascorbate elimination methods: ferric ions, boric acid, nitrites, and ascorbate oxidase (EC 1.10.3.3).
- Analyzed a urine pool with added ascorbate to assess method effectiveness.
- Evaluated features, advantages, and drawbacks of each procedure.
Main Results:
- Boric acid dilution demonstrated effectiveness as a routine method.
- Pre-incubation with ascorbate oxidase reliably prevented ascorbate interference.
- Nitrite treatment was also investigated as a potential alternative.
Conclusions:
- Boric acid dilution is a suitable method for routine urinary oxalate determination.
- Ascorbate oxidase offers a reliable approach to prevent ascorbate interference in oxalate assays.