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A supplementary coil for ²H decoupling with commercial HCN MAS probes
Matthias Huber1, Oliver With, Paul Schanda
1Physical Chemistry, ETH Zürich, Wolfgang-Pauli-Strasse 10, 8093 Zürich, Switzerland.
Abstract:
Partial deuteration is a powerful tool to increase coherence life times and spectral resolution in proton solid-state NMR. The J coupling to deuterium needs, however, to be decoupled to maintain the good resolution in the (usually indirect) (13)C dimension(s). We present a simple and reversible way to expand a commercial 1.3mm HCN MAS probe with a (2)H channel with sufficient field strength for J-decoupling of deuterium, namely 2-3kHz. The coil is placed at the outside of the stator and requires no significant modifications to the probe. The performance and the realizable gains in sensitivity and resolution are demonstrated using perdeuterated ubiquitin, with selectively CHD(2)-labeled methyl groups.
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