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The V protein of mumps virus plays a critical role in pathogenesis

Pei Xu1, Priya Luthra, Zhuo Li

  • 1Department of Infectious Diseases, College of Veterinary Medicine, University of Georgia, Athens, Georgia, USA.

Journal of Virology
|November 18, 2011
PubMed

Insights

Mumps virus (MuV) V protein blocks interferon and IL-6 signaling. Removing V protein (rMuVΔV) from a clinical isolate enhanced IL-6 and reduced viral virulence, highlighting V

Area of Science:

  • Virology
  • Immunology

Background:

  • Mumps virus (MuV) causes diverse symptoms, from parotitis to meningitis.
  • Mumps outbreaks persist despite widespread vaccination.
  • The MuV V protein inhibits interferon (IFN) and interleukin-6 (IL-6) signaling.

Purpose of the Study:

  • To investigate the role of the MuV V protein in viral pathogenesis.
  • To generate and characterize a recombinant MuV lacking V protein expression (rMuVΔV).

Main Methods:

  • Generated a recombinant MuV (rMuVΔV) from a wild-type US isolate (MuV(Iowa/US/06)).
  • Analyzed the impact of V protein deletion on IFN and IL-6 signaling in vitro.
  • Assessed the virulence of rMuVΔV in vivo.

Main Results:

  • The V protein was confirmed to block IFN and IL-6 signaling.
  • rMuVΔV induced significantly higher IL-6 expression in vitro.
  • rMuVΔV exhibited marked attenuation in vivo, demonstrating the V protein's essential role in virulence.

Conclusions:

  • The MuV V protein is crucial for viral virulence.
  • The V protein suppresses IL-6 expression and contributes to pathogenesis.

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