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Updated: May 27, 2026

Combining Fluidic Devices with Microscopy and Flow Cytometry to Study Microbial Transport in Porous Media Across Spatial Scales

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Published on: November 25, 2020

Connecting μ-fluidics to electron microscopy.

Simon Kemmerling¹, Jörg Ziegler, Gabriel Schweighauser

¹Center for Cellular Imaging and Nano Analytics (C-CINA), Biozentrum, Universität Basel, Basel, Switzerland.

Journal of Structural Biology

|November 19, 2011

Summary

This summary is machine-generated.

A new method simplifies electron microscopy (EM) grid preparation for analyzing diverse samples. This technique uses microfluidics and robotic patterning for detailed structural and mass analysis of tiny biological specimens.

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Area of Science:

Biophysics
Materials Science
Analytical Chemistry

Background:

Electron microscopy (EM) is crucial for visualizing biological structures at high resolution.
Current EM grid preparation methods can be complex, time-consuming, and require larger sample volumes.
Analyzing heterogeneous samples with preserved native structure remains a challenge.

Purpose of the Study:

To develop a versatile and efficient methodology for electron microscopy grid preparation.
To enable total content sample analysis from minute volumes.
To allow fine-tuning of preparation characteristics for diverse sample types.

Main Methods:

Integration of a microfluidic-dialysis module for sample conditioning (desalting/mixing with negative stain).
Utilization of a robotic writing table for precise micro-patterning of EM grids.
Processing of heterogeneous samples at physiological pH.

Main Results:

Successful preparation of EM grids enabling comprehensive analysis of sample content.
Demonstrated capability to process minute sample volumes effectively.
Achieved fine control over sample preparation for optimal imaging.

Conclusions:

The presented methodology offers a versatile approach to EM grid preparation.
This technique facilitates detailed structural and mass analysis of heterogeneous samples.
The method enhances efficiency and control in preparing samples for electron microscopy.