Related Experiment Video
Updated: May 27, 2026
An Anoxia-starvation Model for Ischemia/Reperfusion in C. elegans
Published on: March 11, 2014
[Development and application of the physical hypoxic models of C. elegans]
Chang-hong Ren1, Ji-ye Zhang, Jin-ping Shi
1Beijing Institute of Radiation Medicine, State Key Laboratory of Proteomics, State Key Laboratory of Medical Neurobiology, Beijing 100850, China.
Objective:
To develop a suitable hypoxic injury model, which is important for revealing pathological molecular mechanism of hypoxia.
Methods:
We focused on C. elegans by treatment with different hypoxic times and systematically observed mortality, movement, Cellular morphology and the related-protein expression of the animals.
Results:
We demonstrated that hypoxia (0.2% partial pressure of oxygen) induced morphological cell defects, and then leading to death of C. elegans. The mortality of C. elegans increased along with hypoxic time, while hypoxia-inducible factor (HIF-1) was significantly up-regulated. In addition, by using neuron-specific transgenic wonns with green fluorescent protein--we observed the neuron-specffic injury caused by hypoxic stress.
Conclusion:
We successfully established an effective, convenient physical hypoxic model of C. elegans, which will facilitate the studies of hypoxic pathology and molecular mechanisms of hypoxic response in the future.
