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[The expression of connective tissue growth factor in mast cells in the development of pulmonary fibrosis]

Xiao-jing Han1, Xiao-ling Chen, Lin Kang

  • 1Department of Pathophysiology, Tong Shan Fengnan Hospital, Tongshan 063300, China.

Zhongguo Ying Yong Sheng Li Xue Za Zhi = Zhongguo Yingyong Shenglixue Zazhi = Chinese Journal of Applied Physiology
|November 22, 2011
PubMed
Abstract

Insights

Connective tissue growth factor (CTGF) is found in mast cells (MCs) within the lungs during the development of bleomycin-induced pulmonary fibrosis. This suggests MCs may promote lung fibrosis.

Area of Science:

  • Pulmonary Medicine
  • Cell Biology
  • Pathology

Background:

  • Pulmonary fibrosis is a progressive lung disease characterized by scarring.
  • Mast cells (MCs) and connective tissue growth factor (CTGF) are implicated in fibrotic processes.

Purpose of the Study:

  • To determine if CTGF is expressed in lung mast cells during bleomycin-induced pulmonary fibrosis.
  • To investigate the role of mast cells in the pathogenesis of lung fibrosis.

Main Methods:

  • Bleomycin (BLM) was administered intratracheally to rats to induce pulmonary fibrosis.
  • Lung tissue was analyzed for hydroxyproline content, mast cell numbers (toluidine blue stain), and CTGF expression (immunohistochemistry).

Main Results:

  • BLM-induced pulmonary fibrosis was confirmed by increased lung hydroxyproline levels.
  • Increased mast cell numbers and CTGF expression were observed in BLM-treated rats compared to controls.
  • CTGF-positive mast cells were identified in fibrotic lung areas of BLM-treated rats.

Conclusions:

  • Connective tissue growth factor is expressed in mast cells in the lungs during pulmonary fibrosis development.
  • Mast cells expressing CTGF may contribute to the progression of lung fibrosis.

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