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Hypoxia Alters miRNAs Levels Involved in Non-Mendelian Inheritance of Autism Spectrum Disorder in Mice

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High-mobility group box 1 expressions in hypoxia-induced damaged mouse islets.

T Itoh¹, S Iwahashi, M Shimoda

¹Baylor Research Institute, Islet Cell Laboratory, Dallas, Texas, USA.

Transplantation Proceedings

|November 22, 2011

Summary

High-mobility group box 1 (HMGB1) is released from damaged islets exposed to hypoxia. Increased HMGB1 levels indicate islet damage and may serve as a marker for islet potency assays.

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Published on: July 11, 2025

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Mechanism of Regulation of Adipocyte Numbers in Adult Organisms Through Differentiation and Apoptosis Homeostasis

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Published on: June 3, 2016

Area of Science:

Cell Biology
Transplantation Immunology
Biochemistry

Background:

Assessing islet quality is crucial for successful transplantation.
High-mobility group box 1 (HMGB1) has been identified in damaged islets.
Islets are susceptible to hypoxic damage during procurement and storage.

Purpose of the Study:

To investigate HMGB1 expression and release in mouse islets subjected to hypoxia.
To determine if HMGB1 can serve as a damage marker for isolated islets.

Main Methods:

Mouse islets were exposed to hypoxic conditions.
HMGB1 expression was analyzed using quantitative real-time PCR, Western blotting, and ELISA.
Islet function was assessed via transplantation into diabetic mice.

Main Results:

Hypoxia induced HMGB1 translocation from the nucleus to the cytoplasm in islets.
HMGB1 messenger RNA (mRNA) levels increased under hypoxic conditions.
Released HMGB1 levels, particularly per hour, were significantly elevated in damaged islets.

Conclusions:

Hypoxia triggers HMGB1 synthesis and release from damaged islets.
Released HMGB1 may function as a valuable biomarker for detecting islet damage.
HMGB1 quantification could be utilized in islet potency assays for transplantation.