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Related Experiment Video

Updated: May 27, 2026

Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor
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An electrochemical aptasensor based on enzyme linked aptamer assay.

De-Wen Zhang1, Cui-Jin Sun, Fang-Ting Zhang

  • 1Beijing National Laboratory for Molecular Sciences (BNLMS), Key Laboratory of Biochemistry and Molecular Engineering, College of Chemistry, Peking University, Beijing 100871, China.

Biosensors & Bioelectronics
|November 22, 2011
PubMed
Summary

This study presents a novel electrochemical aptasensor for sensitive cocaine detection. The enzyme-linked aptamer assay (ELAA) uses aptamer fragments and achieves a low detection limit, offering a promising tool for drug analysis.

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A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

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Area of Science:

  • Biotechnology
  • Electrochemistry
  • Analytical Chemistry

Background:

  • Aptamers are artificial oligonucleic acids with high affinity and specificity for target molecules.
  • Enzyme-linked aptamer assay (ELAA) is a method for detecting analytes using aptamers.
  • Existing methods for cocaine detection can be complex or lack sensitivity.

Purpose of the Study:

  • To develop a sensitive and specific electrochemical aptasensor for cocaine detection.
  • To establish a new configuration for ELAA using a single aptamer sequence for broader applicability.

Main Methods:

  • Constructed an aptasensor by immobilizing one anticocaine aptamer fragment on a gold electrode.
  • Modified the second aptamer fragment with biotin and streptavidin-horseradish peroxidase (SA-HRP).
  • Utilized differential pulse voltammetry (DPV) to measure changes in reduction current upon cocaine binding.

Main Results:

  • The aptasensor demonstrated sensitive detection of cocaine with a dynamic range from 0.1 μM to 50 μM.
  • Achieved a low detection limit of 20 nM (S/N=3).
  • The assay showed high sensitivity and a low background current.

Conclusions:

  • The proposed electrochemical aptasensor is effective for sensitive and specific cocaine detection.
  • The novel ELAA configuration using a single aptamer sequence is adaptable for detecting various targets.