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Towards a rapid molecular diagnostic for melioidosis: Comparison of DNA extraction methods from clinical specimens
Leisha J Richardson1, Mirjam Kaestli, Mark Mayo
1Menzies School of Health Research, Charles Darwin University, Darwin, Australia.
Journal of Microbiological Methods
|November 24, 2011
Abstract:
Optimising DNA extraction from clinical samples for Burkholderia pseudomallei Type III secretion system real-time PCR in suspected melioidosis patients confirmed that urine and sputum are useful diagnostic samples. Direct testing on blood remains problematic; testing DNA extracted from plasma was superior to DNA from whole blood or buffy coat.
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