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Updated: May 27, 2026

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
Published on: December 9, 2013
Individual bioaerosol particle discrimination by multi-photon excited fluorescence
Denis Kiselev1, Luigi Bonacina, Jean-Pierre Wolf
1Université de Genève, GAP-Biophotonics, Rue de l’Ecole de Médecine 20, 1211 Geneva 4, Switzerland. Denis.Kiselev@unige.ch
Abstract:
Femtosecond laser induced multi-photon excited fluorescence (MPEF) from individual airborne particles is tested for the first time for discriminating bioaerosols. The fluorescence spectra, analysed in 32 channels, exhibit a composite character originating from simultaneous two-photon and three-photon excitation at 790 nm. Simulants of bacteria aggregates (clusters of dyed polystyrene microspheres) and different pollen particles (Ragweed, Pecan, Mulberry) are clearly discriminated by their MPEF spectra. This demonstration experiment opens the way to more sophisticated spectroscopic schemes like pump-probe and coherent control.
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