Treatment for chronic ischaemia-induced bladder detrusor dysfunction using bone marrow mesenchymal stem cells: an...

Area of Science:

• Regenerative Medicine
• Urology
• Stem Cell Biology

Background:

• Ischemia-induced bladder dysfunction is poorly understood, potentially involving M-receptor hypersensitivity, nerve injury, and fibrosis.
• Existing treatments for bladder ischemia's histological and functional changes are ineffective.
• Bladder outlet obstruction and aging are linked to chronic bladder ischemia.

Purpose of the Study:

• To evaluate the feasibility and effectiveness of bone marrow mesenchymal stem cells (BM-MSCs) for treating chronic ischemia-induced bladder detrusor dysfunction.
• To assess the impact of BM-MSCs on bladder function and histology in an experimental rat model.

Main Methods:

• BM-MSCs from Sprague-Dawley (SD) rats were injected into the common iliac artery, followed by iliac artery ligation and doxazosin mesylate administration.
• Urodynamic examinations and intravesical pressure measurements were conducted eight weeks post-treatment.
• Histological analysis included immunohistochemistry and trichrome staining to evaluate smooth muscle content and nerve fibers.

Main Results:

• Ischemic bladders showed significantly reduced intravesical pressure and detrusor contraction power compared to experimental and control groups.
• Stem cell treatment (experimental group) restored intravesical pressure and detrusor contraction power, showing no significant difference from the control group.
• Histology revealed increased smooth muscle content and nerve fibers in the experimental group compared to the ischemia group, with stem cell regeneration confirmed by BrdU staining.

Conclusions:

• Chronic bladder ischemia leads to decreased smooth muscle content, reduced nerve fibers, and impaired detrusor contractility.
• Intra-arterial BM-MSC injection combined with doxazosin mesylate promotes stem cell regeneration in ischemic bladder tissue.
• This treatment approach effectively improves bladder detrusor smooth muscle content, nerve regeneration, and overall bladder function.