Related Experiment Video
Updated: May 27, 2026

Measurement of Cyclic Guanosine Monophosphate (cGMP) in Solid Tissues using Competitive Enzyme-Linked Immunosorbent Assay (ELISA)
Published on: July 3, 2025
Regulation of cardiovascular TRP channel functions along the NO-cGMP-PKG axis
Ryuji Inoue1, Juan Shi, Zhong Jian
1Department of Physiology, Graduate School of Medcial Sciences, Fukuoka University, Fukuoka, Japan. inouery@fukuoka-u.ac.jp
Abstract:
There is growing body of evidence that nitric oxide (NO)-cGMP-PKG signaling plays a central role in negative regulation of cardiovascular (CV) responses and its disorders through suppressed Ca(2+) dynamics. Other lines of evidence also reveal the stimulatory effects of this signaling on some CV functions. Recently, transient receptor potential (TRP) channels have received much attention as non-voltage-gated Ca(2+) channels involved in CV physiology and pathophysiology. Available information suggests that these channels undergo both inhibition and activation by NO via PKG-mediated phosphorylation and S-nitrosylation, respectively, and also act as upstream regulators to promote endothelial NO production. This review summarizes the roles of NO-cGMP-PKG signaling pathway, particularly in regulating TRP channel functions with their associated physiology and pathophysiology.
More Related Videos
08:27Expression and Purification of the Human Lipid-sensitive Cation Channel TRPC3 for Structural Determination by Single-particle Cryo-electron Microscopy
Published on: January 7, 2019
07:51Mesenteric Artery Contraction and Relaxation Studies Using Automated Wire Myography
Published on: September 22, 2011
Related Concept Videos
G-Protein Gated Ion Channels
Sensory organs,...
Nitric Oxide Signaling Pathway
GPCRs Regulate Adenylyl Cylase Activity
Two...
Activation and Inactivation of G Proteins
Transducer Mechanism: Enzyme-Linked Receptors
Major types that are helpful drug targets include:
Calmodulin-dependent Signaling
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...