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Staphylococccus pseudintermedius surface proteins SpsD and SpsO mediate adherence to ex vivo canine corneocytes

Jeanette Bannoehr1, Jeremy K Brown, Darren J Shaw

  • 1Dermatology Unit, The Roslin Institute and The Royal (Dick) School of Veterinary Studies, The University of Edinburgh, Easter Bush Veterinary Centre, Roslin, Midlothian EH25 9RG, UK. jeanette.bannoehr@vetsuisse.unibe.ch

Veterinary Dermatology
|November 25, 2011
PubMed

Insights

Two Staphylococcus pseudintermedius surface proteins, SpsD and SpsO, were found to mediate bacterial adherence to canine skin cells. These findings offer potential new therapeutic targets for treating canine pyoderma.

Area of Science:

  • Veterinary Microbiology
  • Bacterial Pathogenesis
  • Molecular Biology

Background:

  • Staphylococcus pseudintermedius is a primary cause of canine pyoderma.
  • Limited understanding exists regarding the specific bacterial virulence factors contributing to canine pyoderma.
  • Previous genomic analysis identified 18 potential cell-wall-anchored surface proteins (SpsA-SpsR) in S. pseudintermedius.

Purpose of the Study:

  • To investigate the role of three specific S. pseudintermedius surface proteins (SpsD, SpsL, SpsO) in bacterial adherence to canine corneocytes.
  • To identify potential virulence factors that contribute to canine pyoderma pathogenesis.

Main Methods:

  • Expressed SpsD, SpsL, and SpsO on the surface of Lactococcus lactis, a non-adherent surrogate host.
  • Performed adherence assays using corneocytes from five healthy canine donors.
  • Quantified bacterial adherence via computerized image analysis.

Main Results:

  • SpsD and SpsO significantly mediated the adherence of L. lactis to canine corneocytes.
  • SpsL did not demonstrate a significant role in adherence under the tested conditions.
  • These results highlight SpsD and SpsO as key factors in S. pseudintermedius adherence.

Conclusions:

  • SpsD and SpsO are identified as crucial bacterial virulence factors involved in canine pyoderma.
  • These proteins represent promising novel therapeutic targets for combating Staphylococcus pseudintermedius infections in dogs.

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