Related Experiment Video
Updated: May 27, 2026

Combined Immunofluorescence and DNA FISH on 3D-preserved Interphase Nuclei to Study Changes in 3D Nuclear Organization
Published on: February 3, 2013
A simple method for the immunocytochemical detection of proteins inside nuclear structures that are inaccessible to
Darya M Svistunova1, Yana R Musinova, Vladimir Yu Polyakov
1AN Belozersky Institute of Physico-Chemical Biology, Moscow State University, Moscow, Russia.
Abstract:
It has been demonstrated elsewhere that a high concentration of an antigen within the nucleolus may prevent its proper recognition by specific antibodies. In this study, the authors found that a short proteinase treatment allowed for the detection of antigens in the nucleoli. The described approach is compatible with the simultaneous observation of proteins fused to fluorescent tags and with preembedding electron microscopy. It appears that the described method can be useful in situations when the proper recognition of antigens by specific antibodies is disturbed by a high density of cellular structures or a high concentration of antigens inside these structures.
Related Concept Videos
Immunogold Electron Microscopy
Immunocytochemistry and Immunohistochemistry
These...
Immunofluorescence Microscopy
The...

