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Published on: August 15, 2016
Investigation of the inclusion behavior of chlorogenic acid with hydroxypropyl-β-cyclodextrin
Jianbin Chao1, Hongfang Wang, Wei Zhao
1Research Institute of Applied Chemistry, Shanxi University, Taiyuan, PR China. chao@sxu.edu.cn
Abstract:
The inclusion complexation behavior of chlorogenic acid (CGA) with the hydroxypropyl-β-cyclodextrin (HP-β-CD) was investigated in both solution and the solid state by UV-vis and fluorescence spectroscopy, infrared spectroscopy (IR), NMR spectroscopy as well as differential scanning calorimetry (DSC). The experimental results indicate that CGA is able to form an inclusion complex with HP-β-CD. The inclusion complex has a stoichiometry of 1:1 and the formation constant was calculated to be 155.7 M(-1). The antioxidant activity of CGA on complexation with HP-β-CD increased as compared to uncomplexed CGA. NMR spectroscopic studies show that the aromatic ring and the vinyl group of CGA are deeply included inside the CD cavity.
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