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Macromolecular release from collagen monolithic devices.

D L Gilbert1, S W Kim

  • 1Department of Pharmaceutics, University of Utah, Salt Lake City, 84108.

Journal of Biomedical Materials Research
|September 1, 1990
PubMed
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This study shows that collagen devices with varying crosslinking controlled inulin release and degradation. Collagen matrices demonstrated good biocompatibility and slow degradation in vivo, suggesting potential for controlled drug delivery.

Area of Science:

  • Biomaterials Science
  • Drug Delivery Systems
  • Tissue Engineering

Background:

  • Collagen-based materials are widely explored for biomedical applications due to their biocompatibility.
  • Controlling the release and degradation of collagen devices is crucial for effective therapeutic outcomes.
  • Understanding the influence of fabrication parameters on device performance is essential for optimizing their use.

Purpose of the Study:

  • To investigate the impact of crosslinking density, collagen structure, and crosslinker type on the in vitro release kinetics and biodegradation of collagen monolithic devices.
  • To evaluate the in vivo biocompatibility, degradation profile, and drug release characteristics of these collagen devices in a subcutaneous rat model.

Main Methods:

  • Fabrication of collagen monolithic devices with varied crosslinking densities, structures, and crosslinkers.

Related Experiment Videos

  • In vitro release studies using inulin as a model macromolecule.
  • In vitro biodegradation assays using proteolytic enzymes, including collagenase.
  • In vivo subcutaneous implantation in rats to assess biocompatibility, degradation, and 14C-inulin release.

    • Main Results:

    • In vitro inulin release rates were linear with t1/2 and significantly influenced by crosslinking parameters and collagen structure.
    • Collagen devices were resistant to degradation by proteolytic enzymes, with collagenase degradation dependent on device properties and enzyme concentration.
    • In vivo studies showed no severe cellular response to collagen discs after 3 weeks, with minimal degradation observed.
    • Dacron implants elicited a stronger fibroblast response but fewer inflammatory cells compared to collagen.
    • In vivo release of 14C-inulin from collagen devices was diffusion-controlled.

    Conclusions:

    • Collagen monolithic devices can be fabricated with tunable properties to control macromolecule release and degradation.
    • The crosslinking strategy and collagen structure are key determinants of both in vitro and in vivo performance.
    • Collagen devices exhibit favorable biocompatibility and stability in vivo, supporting their potential for controlled drug delivery applications.