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Related Concept Videos

Chromosome Replication02:31

Chromosome Replication

Before a cell can divide, it must accurately replicate all of its chromosomes, including the DNA and its associated histone and non-histone proteins.  This process begins at numerous origins of replication during the S phase of the cell cycle in each of a cell’s chromosomes simultaneously. Certain nucleotides can act as origins of replication, but these sequences are not well defined - especially in complex, multi-cellular, eukaryotic species. The length of DNA that spans an origin of...
Replication in Eukaryotes01:29

Replication in Eukaryotes

In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...
Replication in Eukaryotes02:31

Replication in Eukaryotes

Overview
Replication in Eukaryotes02:31

Replication in Eukaryotes

Overview
Replication in Eukaryotes01:29

Replication in Eukaryotes

In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...
Replication in Prokaryotes01:32

Replication in Prokaryotes

DNA replication has three main steps: initiation, elongation, and termination. Replication in prokaryotes begins when initiator proteins bind to the single origin of replication (ori) on the cell's circular chromosome. Replication then proceeds around the entire circle of the chromosome in each direction from the two replication forks, resulting in two DNA molecules.
Many Proteins Work Together to Replicate the Chromosome
Replication is coordinated and carried out by a host of specialized...

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Related Experiment Video

Updated: May 27, 2026

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome
06:40

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

Published on: March 22, 2018

OriDB, the DNA replication origin database updated and extended.

Cheuk C Siow1, Sian R Nieduszynska, Carolin A Müller

  • 1Centre for Genetics and Genomics, The University of Nottingham, Queen's Medical Centre, Nottingham NG7 2UH, UK.

Nucleic Acids Research
|November 29, 2011
PubMed
Summary
This summary is machine-generated.

The OriDB database now includes replication origin mapping data for both budding yeast (Saccharomyces cerevisiae) and fission yeast (Schizosaccharomyces pombe), enhancing accessibility and expanding curated studies for researchers.

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Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method
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Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method

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Related Experiment Videos

Last Updated: May 27, 2026

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome
06:40

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

Published on: March 22, 2018

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method
08:53

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method

Published on: May 2, 2025

Area of Science:

  • Genetics
  • Genomics
  • Molecular Biology

Background:

  • Replication origins are crucial for DNA replication initiation.
  • Genome-wide mapping studies identify these origin sites.
  • Databases consolidate this information for research.

Purpose of the Study:

  • To revamp the OriDB database for improved user accessibility.
  • To expand curated replication origin mapping studies.
  • To include origin data for Schizosaccharomyces pombe.

Main Methods:

  • Collating and curating genome-wide mapping studies.
  • Revamping database structure for enhanced data management.
  • Integrating data for Saccharomyces cerevisiae and Schizosaccharomyces pombe.

Main Results:

  • OriDB now offers expanded, curated origin mapping data.
  • User accessibility to datasets has been significantly improved.
  • Replication origin sites for S. pombe are now included.

Conclusions:

  • The updated OriDB facilitates research on DNA replication origins.
  • The revamped database structure supports future expansion.
  • OriDB serves as a valuable resource for yeast genomics.