Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Bacterial Transformation01:33

Bacterial Transformation

In 1928, bacteriologist Frederick Griffith worked on a vaccine for pneumonia, which is caused by Streptococcus pneumoniae bacteria. Griffith studied two pneumonia strains in mice: one pathogenic and one non-pathogenic. Only the pathogenic strain killed host mice.
Griffith made an unexpected discovery when he killed the pathogenic strain and mixed its remains with the live, non-pathogenic strain. Not only did the mixture kill host mice, but it also contained living pathogenic bacteria that...
Bacterial Transformation01:33

Bacterial Transformation

In 1928, bacteriologist Frederick Griffith worked on a vaccine for pneumonia, which is caused by Streptococcus pneumoniae bacteria. Griffith studied two pneumonia strains in mice: one pathogenic and one non-pathogenic. Only the pathogenic strain killed host mice.
Griffith made an unexpected discovery when he killed the pathogenic strain and mixed its remains with the live, non-pathogenic strain. Not only did the mixture kill host mice, but it also contained living pathogenic bacteria that...
Genome Size and the Evolution of New Genes03:21

Genome Size and the Evolution of New Genes

While every living organism has a genome of some kind (be it RNA, or DNA), there is considerable variation in the sizes of these blueprints. One major factor that impacts genome size is whether the organism is prokaryotic or eukaryotic. In prokaryotes, the genome contains little to no non-coding sequence, such that genes are tightly clustered in groups or operons sequentially along the chromosome. Conversely, the genes in eukaryotes are punctuated by long stretches of non-coding sequence.
Antibiotic Selection00:57

Antibiotic Selection

Overview
Transduction01:16

Transduction

Among the three main modes of HGT—transformation, conjugation, and transduction—transduction is unique in that it is mediated by bacteriophages, or bacterial viruses.Transduction occurs in two ways. Generalized transduction occurs during the lytic cycle of a bacteriophage infection. In this process, bacteriophages infect bacterial cells, replicate within them, and ultimately cause cell lysis, releasing newly assembled virions. Occasionally, random fragments of the bacterial genome are...
Conjugation01:19

Conjugation

Conjugation is a form of horizontal gene transfer that primarily occurs in bacteria and some archaea, promoting genetic diversity and adaptation. Bacteria can acquire resistance genes through conjugative plasmids, allowing them to survive antibiotic treatments that would otherwise be lethal. This process involves direct contact between cells through specialized structures such as the sex pilus and is mediated by conjugative plasmids, including the F (fertility) factor.Conjugation requires...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A single viral enzyme drives tRNA-dependent hypermodification of DNA at adenine.

Nature communications·2026
Same author

Biology of host-dependent restriction-modification in prokaryotes.

EcoSal Plus·2025
Same author

Reassembling a cannon in the DNA defense arsenal: Genetics of StySA, a BREX phage exclusion system in Salmonella lab strains.

PLoS genetics·2022
Same author

Genome archaeology of two laboratory Salmonella enterica enterica sv Typhimurium.

G3 (Bethesda, Md.)·2021
Same author

Complete Annotated Genome Sequence of the Salmonella enterica Serovar Typhimurium LT7 Strain STK003, Historically Used in Gene Transfer Studies.

Microbiology resource announcements·2021
Same author

Genome analysis of Salmonella enterica serovar Typhimurium bacteriophage L, indicator for StySA (StyLT2III) restriction-modification system action.

G3 (Bethesda, Md.)·2021
Same journal

Correction to 'scSuperAnnotator: A platform for benchmarking comparison and visualizing automated cellular annotation methods for scRNA-seq data'.

Nucleic acids research·2026
Same journal

Correction to 'Differentiable partition function calculation for RNA'.

Nucleic acids research·2026
Same journal

Deployment of non-canonical splicing in tunicate genomes is mediated by divergent U2AF function and changing m6A modification in U1 and U6 snRNA.

Nucleic acids research·2026
Same journal

Bacillus subtilis DnaB forms multiple protein-protein interactions essential for DNA replication initiation.

Nucleic acids research·2026
Same journal

Multiple forms of protein-protein and DNA binding are exhibited by BrxC from the BREX phage restriction system.

Nucleic acids research·2026
Same journal

Biosynthesis of glycosylated 5-hydroxycytosine in the DNA of diverse viruses.

Nucleic acids research·2026
See all related articles

Related Experiment Video

Updated: May 27, 2026

Site-specific Bacterial Chromosome Engineering: ΦC31 Integrase Mediated Cassette Exchange (IMCE)
08:21

Site-specific Bacterial Chromosome Engineering: ΦC31 Integrase Mediated Cassette Exchange (IMCE)

Published on: March 16, 2012

A versatile element for gene addition in bacterial chromosomes.

Marion H Sibley1, Elisabeth A Raleigh

  • 1New England Biolabs, 240 County Road, Ipswich, MA 01938, USA.

Nucleic Acids Research
|November 30, 2011
PubMed
Summary
This summary is machine-generated.

This study introduces a rapid, marker-free method for bacterial gene insertion using a novel vector. The system offers precise control over gene expression, enabling efficient genetic manipulation for various applications.

More Related Videos

Determination of the Optimal Chromosomal Location(s) for a DNA Element in Escherichia coli Using a Novel Transposon-mediated Approach
11:12

Determination of the Optimal Chromosomal Location(s) for a DNA Element in Escherichia coli Using a Novel Transposon-mediated Approach

Published on: September 11, 2017

Characterizing Multidrug Efflux Systems in Acinetobacter baumannii Using an Efflux-Deficient Bacterial Strain and a Single-Copy Gene Expression System
05:06

Characterizing Multidrug Efflux Systems in Acinetobacter baumannii Using an Efflux-Deficient Bacterial Strain and a Single-Copy Gene Expression System

Published on: January 5, 2024

Related Experiment Videos

Last Updated: May 27, 2026

Site-specific Bacterial Chromosome Engineering: ΦC31 Integrase Mediated Cassette Exchange (IMCE)
08:21

Site-specific Bacterial Chromosome Engineering: ΦC31 Integrase Mediated Cassette Exchange (IMCE)

Published on: March 16, 2012

Determination of the Optimal Chromosomal Location(s) for a DNA Element in Escherichia coli Using a Novel Transposon-mediated Approach
11:12

Determination of the Optimal Chromosomal Location(s) for a DNA Element in Escherichia coli Using a Novel Transposon-mediated Approach

Published on: September 11, 2017

Characterizing Multidrug Efflux Systems in Acinetobacter baumannii Using an Efflux-Deficient Bacterial Strain and a Single-Copy Gene Expression System
05:06

Characterizing Multidrug Efflux Systems in Acinetobacter baumannii Using an Efflux-Deficient Bacterial Strain and a Single-Copy Gene Expression System

Published on: January 5, 2024

Area of Science:

  • Synthetic Biology
  • Bacterial Genetics
  • Metabolic Engineering

Background:

  • Genetic manipulation is crucial for optimizing bacterial metabolic pathways for producing proteins and small molecules.
  • Existing methods for gene insertion into bacterial chromosomes often leave behind selectable markers and can be time-consuming.
  • There is a need for efficient, marker-free gene integration techniques for streamlined genetic engineering.

Purpose of the Study:

  • To develop a novel vector and a rapid four-day procedure for site-specific chromosomal gene insertion in bacteria.
  • To establish a gene expression system that is independently regulated by rhamnose, avoiding common inducers like IPTG and arabinose.
  • To demonstrate the utility of this system for controlled gene expression and genetic construction series.

Main Methods:

  • Development of a specialized vector for site-specific chromosomal insertion.
  • Implementation of a four-day protocol for gene integration.
  • Utilizing the rhamnose-inducible promoter (rhaBp) for gene expression control.
  • Employing lacZ as a reporter gene to assess promoter regulation.
  • Using a DNA methyltransferase to evaluate on-off gene expression control.

Main Results:

  • The rhamnose-inducible promoter (rhaBp) demonstrated tight regulation with minimal background expression and moderate induced expression compared to IPTG-induced systems.
  • Expression of a DNA methyltransferase under rhamnose control showed clear on-off switching, impacting plasmid sensitivity to restriction enzymes.
  • Growth medium manipulation allowed for fine-tuning of gene expression levels.
  • The vector proved adaptable for open reading frame (ORF) cloning.

Conclusions:

  • The developed vector and procedure enable quick, marker-free, site-specific gene insertion into bacterial chromosomes.
  • Rhamnose-inducible gene expression offers precise, independent regulation, suitable for complex genetic constructions.
  • This system enhances bacterial genetic engineering capabilities for protein and small molecule production.