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Updated: May 27, 2026

Quantification of Site-specific Protein Lysine Acetylation and Succinylation Stoichiometry Using Data-independent Acquisition Mass Spectrometry
Published on: April 4, 2018
Analysis of underivatized amino acids: zwitterionic hydrophilic interaction chromatography combined with triple
Madeleine Dell'mour1, Gunda Koellensperger, Stephan Hann
1Department of Chemistry, University of Natural Resources and Life Sciences - BOKU Vienna, Vienna, Austria.
Abstract:
Analysis of underivatized amino acids is challenging regarding both separation and detection of this small, polar, and largely UV-inactive compounds. Additives for reversed phase chromatography such as ion-pairing reagents can hamper mass spectrometric detection. Zwitterionic hydrophilic interaction chromatography using MS compatible eluents together with tandem mass spectrometry in multiple reaction monitoring mode for selective detection is an attractive approach to overcome the above mentioned issues.
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