Related Experiment Video
Updated: May 27, 2026

Using the E1A Minigene Tool to Study mRNA Splicing Changes
Published on: April 22, 2021
Analysis of isoform expression from splicing array using multiple comparisons
1Departments of Biological Sciences, Computer Science/Informatics, Indiana University South Bend, Bloomington, IN, USA. mnair@iusb.edu
Abstract:
There is a high prevalence of alternatively spliced genes (isoforms) in the human genome. Studies toward understanding aberrantly spliced genes and their association with diseases have lead researchers to profile the expression of alternatively spliced products. High-throughput profiling of isoforms has been done using microarray technology. Expression of isoforms reflects regulation both at transcriptional and posttranscriptional levels. This chapter details the methods to perform exhaustive comparison of isoforms using the R statistical framework.
Related Concept Videos
Comparing Copy Number Variations and SNPs
Copy number variations or CNVs are the structural variations that cover more than 1kb of DNA sequence. The single nucleotide polymorphism (SNP), on the other hand, is a single nucleotide change or a point mutation that is found in more than 1%...
Alternative RNA Splicing
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Alternative RNA Splicing
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
RNA Splicing
RNA Splicing
Pre-mRNA Processing: RNA Splicing

