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Updated: May 27, 2026

Immunoblot Analysis
16:01

Immunoblot Analysis

Published on: June 20, 2008

Analysis of signalling pathways by western blotting and immunoprecipitation

Aymen I Idris1

  • 1Bone and Cancer Group, Edinburgh Cancer Research UK Centre and Rheumatic Disease Unit, the Centre of Molecular Medicine, University of Edinburgh, Edinburgh, UK. aymen.idris@ed.ac.uk

Methods in Molecular Biology (Clifton, N.J.)
|December 2, 2011
PubMed

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Western Blotting01:15

Western Blotting

Western blotting is an analytical technique for protein identification. It has various applications in immunology and medicine, including detecting diseases like bovine spongiform encephalopathy, mad cow disease, and human and feline immunodeficiency virus from biological samples.
The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.