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Related Concept Videos

Gross Anatomy of Bone01:17

Gross Anatomy of Bone

The two main features of a long bone are the diaphysis and the epiphysis.
The diaphysis is the tubular shaft that runs between the proximal and distal ends of the bone. The walls of the diaphysis are composed of dense and hard compact bone made of numerous osteons — the functional unit of the compact bone. The hollow region in the diaphysis is called the medullary cavity, which harbors the bone marrow. In infants and children, this marrow cavity is filled with red marrow, whereas in adults, it...
Compact Bone01:27

Compact Bone

Most bones contain compact and spongy osseous tissue, but their distribution and concentration vary based on the bone's overall function.
Compact bone, also called cortical bone, is the denser, stronger of the two types of bone tissue. It is found under the periosteum and in the diaphyses of long bones, where it provides support and protection. The microscopic structural unit of compact bone is called an osteon, or haversian system. Each osteon is composed of concentric rings of calcified...
Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and the...
Spongy Bone01:09

Spongy Bone

All bones comprise an outer layer of compact bone, and an interior made up of spongy bone tissue, also called cancellous or trabecular bone. In long bones, spongy bone tissue is mainly found in the interior of the epiphyses (broad ends of the bone).
Spongy bone is more porous, and less dense compared to compact bone. It is composed of concentric lamellae that are arranged irregularly to form the trabecular network. In some bones, the spaces between trabeculae contain red marrow, where...
Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...

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Multimodal Approach to Assess Bone Regeneration and Scaffold Performance
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Raman microscopy of bone.

Simon R Goodyear1, Richard M Aspden

  • 1Musculoskeletal Research Programme, Division of Applied Medicine, Institute of Medical Sciences, University of Aberdeen, Aberdeen, UK. s.goodyear@abdn.ac.uk

Methods in Molecular Biology (Clifton, N.J.)
|December 2, 2011
PubMed
Summary
This summary is machine-generated.

Raman microscopy offers a non-destructive way to analyze bone

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Area of Science:

  • Biomaterials Science
  • Biophysics
  • Materials Science

Background:

  • Bone is a complex composite material primarily composed of mineral and collagen.
  • Understanding the chemical properties of these components is crucial for bone research.
  • Characterizing bone requires techniques that can analyze its structure at a microscopic level.

Purpose of the Study:

  • To highlight Raman microscopy as a valuable tool for bone analysis.
  • To demonstrate its capability in simultaneously measuring mineral and collagen in bone.
  • To emphasize its advantages for bone characterization.

Main Methods:

  • Utilizing Raman microscopy, a non-destructive technique.
  • Employing modern Raman instruments with integrated software.
  • Achieving spatial resolution of approximately one micron.

Main Results:

  • Raman microscopy enables simultaneous measurement of bone's mineral and collagen.
  • The technique requires minimal sample preparation.
  • Standard information required for bone studies can be acquired efficiently.

Conclusions:

  • Raman microscopy is a powerful, non-destructive method for bone chemical analysis.
  • Its high spatial resolution and minimal sample requirements make it ideal for bone studies.
  • It serves as a valuable addition to the bone characterization toolbox.