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Proteomics

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...

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Updated: May 27, 2026

Fast Enzymatic Processing of Proteins for MS Detection with a Flow-through Microreactor

Fast Enzymatic Processing of Proteins for MS Detection with a Flow-through Microreactor

Published on: April 6, 2016

Limited proteolysis in proteomics using protease-immobilized microreactors.

Hiroshi Yamaguchi¹, Masaya Miyazaki, Hideaki Maeda

¹Measurement Solution Research Center, National Institute of Advanced Industrial Science and Technology, Tosu, Saga, Japan.

Methods in Molecular Biology (Clifton, N.J.)

|December 2, 2011

Summary

This summary is machine-generated.

Protease-immobilized microreactors offer rapid, efficient proteolysis for mass spectrometry (MS) analysis. This method simplifies sample preparation and enhances enzyme stability, improving proteomic workflows.

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Last Updated: May 27, 2026

Fast Enzymatic Processing of Proteins for MS Detection with a Flow-through Microreactor

Fast Enzymatic Processing of Proteins for MS Detection with a Flow-through Microreactor

Published on: April 6, 2016

Digital Microfluidics for Automated Proteomic Processing

Digital Microfluidics for Automated Proteomic Processing

Published on: November 6, 2009

Evaluation of Protein–Protein Interactions using an On-Membrane Digestion Technique

Evaluation of Protein–Protein Interactions using an On-Membrane Digestion Technique

Published on: July 19, 2019

Area of Science:

Biochemistry
Analytical Chemistry
Proteomics

Background:

Proteolysis is crucial for mass spectrometry (MS)-based proteomic studies.
Conventional in-solution digestion methods present challenges like time consumption and purification steps.
Enzyme stability and workflow efficiency are key considerations in proteomic sample preparation.

Purpose of the Study:

To describe the preparation of protease-immobilized microreactors.
To evaluate the proteolysis performance of these microreactors.
To highlight the advantages of microreactor-based proteolysis for proteomic analysis.

Main Methods:

Immobilization of proteases onto the inner wall of microchannels via a polymeric membrane of protease-proteins.
Utilizing cross-linking reactions between lysine residues in laminar flow for immobilization.
Employing poly-lysine for acidic proteins prior to cross-linking.

Main Results:

Protease-immobilized microreactors enable rapid and efficient proteolysis.
The method eliminates the need for purification of digests before MS analysis.
Microreactors demonstrate high stability against chemical and thermal denaturants.

Conclusions:

Protease-immobilized microreactors offer a simplified and efficient approach to proteolysis for MS.
This technique enhances enzyme stability and streamlines proteomic workflows.
The immobilization procedure is versatile and applicable to various enzymes beyond proteases.