Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Microbial Biosensors

Microbial Biosensors

Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...

Overview Of Cell Separation And Isolation

Overview Of Cell Separation And Isolation

Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Corrigendum to "Fully-printed microneedles meet plants: a pathway towards easy-to-use NFC monitoring of total ionic conductivity in precision agriculture" [Biosens. Bioelectron. 306 (2026) 118667].

Biosensors & bioelectronics·2026

Same author

A paper-based fluorescent biosensing platform for miRNA detection with dual high-throughput and smartphone readout.

Biosensors & bioelectronics·2026

Same author

Fully-printed microneedles meet plants: a pathway towards easy-to-use NFC monitoring of total ionic conductivity in precision agriculture.

Biosensors & bioelectronics·2026

Same author

Laser-Assisted In Situ Fabrication of rGO/Bi<sub>2</sub>O<sub>3</sub> Nanocomposites for Heavy Metal Sensing.

Small (Weinheim an der Bergstrasse, Germany)·2026

Same author

Built-in thiol mesoporous carbon immunosensor to detect carcinoembryonic antigen in human serum.

Mikrochimica acta·2026

Same author

Beta 2 adrenergic receptor agonists as a treatment for metabolic dysfunction-associated steatohepatitis (MASH).

npj metabolic health and disease·2026

Same journal

Monolithic Axial InGaAs Quantum Dot Emitters in GaAs-Based Nanowires via Sb-Mediated Facet Engineering.

Nano letters·2026

Same journal

Electrical Imaging of DNA Substructures Using Quasi-Static Nanopore Scanning.

Nano letters·2026

Same journal

Structural Basis of Hemoglobin Amyloid Fibrils Revealed by cryo-EM and Molecular Dynamics Simulations.

Nano letters·2026

Same journal

Rashba-Related Spin-Selective Effect in 2D Chiral Perovskites with Achiral Organic Cation Spacers.

Nano letters·2026

Same journal

Visualizing Superconducting Gap Modulation Induced by Pair-Breaking Scattering Interference in Bulk FeSe.

Nano letters·2026

Same journal

Generalized Geometric Phase for Coupled Meta-Atoms.

Nano letters·2026

See all related articles

Search research articles

Related Experiment Video

Updated: May 27, 2026

Photodegradable Hydrogel Interfaces for Bacteria Screening, Selection, and Isolation

Photodegradable Hydrogel Interfaces for Bacteria Screening, Selection, and Isolation

Published on: November 4, 2021

Bacterial isolation by lectin-modified microengines.

Susana Campuzano¹, Jahir Orozco, Daniel Kagan

¹Department of Nanoengineering, University of California-San Diego, La Jolla, California 92093, USA.

|December 6, 2011

Summary

This summary is machine-generated.

New self-propelled microtubular engines efficiently isolate Escherichia coli (E. coli) bacteria in real-time. These advanced microengines also capture drug particles, offering a novel theranostics approach for diagnostics and treatment.

More Related Videos

Engineering Adherent Bacteria by Creating a Single Synthetic Curli Operon

Engineering Adherent Bacteria by Creating a Single Synthetic Curli Operon

Published on: November 16, 2012

Scalable Isolation and Purification of Extracellular Vesicles from Escherichia coli and Other Bacteria

Scalable Isolation and Purification of Extracellular Vesicles from Escherichia coli and Other Bacteria

Published on: October 13, 2021

Related Experiment Videos

Last Updated: May 27, 2026

Photodegradable Hydrogel Interfaces for Bacteria Screening, Selection, and Isolation

Photodegradable Hydrogel Interfaces for Bacteria Screening, Selection, and Isolation

Published on: November 4, 2021

Engineering Adherent Bacteria by Creating a Single Synthetic Curli Operon

Engineering Adherent Bacteria by Creating a Single Synthetic Curli Operon

Published on: November 16, 2012

Scalable Isolation and Purification of Extracellular Vesicles from Escherichia coli and Other Bacteria

Scalable Isolation and Purification of Extracellular Vesicles from Escherichia coli and Other Bacteria

Published on: October 13, 2021

Area of Science:

Nanotechnology
Biotechnology
Microfluidics

Background:

Escherichia coli (E. coli) poses significant risks in environmental, food, and clinical settings.
Rapid and real-time detection of E. coli is crucial for public health.
Current methods for bacterial isolation can be time-consuming and lack multifunctionality.

Purpose of the Study:

To develop novel self-propelled microtubular engines for rapid E. coli isolation.
To integrate selective bacterial capture with drug delivery capabilities.
To demonstrate a motion-based theranostics strategy for E. coli detection and treatment.

Main Methods:

Fabrication of template-based gold/nickel/polyaniline/platinum (Au/Ni/PANI/Pt) microtubular engines.
Functionalization of microtubes with Concanavalin A (ConA) lectin bioreceptors for E. coli targeting.
Utilizing microtube propulsion for bacteria capture and drug-carrier particle uptake.
Demonstrating triggered release of captured bacteria in a low-pH glycine solution.

Main Results:

The Au/Ni/PANI/Pt microtubular engines demonstrated efficient and rapid isolation of E. coli.
The microengines successfully combined E. coli capture with the uptake of polymeric drug-carrier particles.
Triggered release of captured E. coli was achieved by navigating the microengines through a specific dissociation solution.
The small size of the microengines enabled label-free optical visualization and discrimination against non-target cells.

Conclusions:

The developed ConA-functionalized Au/Ni/PANI/Pt microtubular engines represent a significant advancement in E. coli isolation technology.
These multifunctional microengines offer a promising platform for motion-based theranostics, combining diagnostics and therapeutics.
The technology facilitates convenient, real-time, and label-free detection and potential treatment of bacterial infections.