Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

T Cell Types and Functions01:24

T Cell Types and Functions

When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
Receptor Downregulation in MVBs01:15

Receptor Downregulation in MVBs

Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded.
The EGFR can initiate signaling pathways that  lead to cell proliferation, migration, and differentiation. Overexpression of EGFR  stimulates cells to proliferate. Excessive  EGFR activation may...
The JAK-STAT Signaling Pathway01:20

The JAK-STAT Signaling Pathway

Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as  SH2...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The efficacy of immunotherapy in glioma requires distal B cell responses in tumor-draining lymph nodes.

Science immunology·2026
Same author

The EphA3 Antibody Enhances CD47 Antibody-Mediated Phagocytosis by Repolarizing M2-Like Tumor-Associated Macrophages in a Preclinical Model of Recurrent Head and Neck Cancer.

OncoTargets and therapy·2026
Same author

TonEBP as a key regulator of hypothalamic leptin signaling and resistance.

Cellular and molecular life sciences : CMLS·2026
Same author

Physiological Changes and Transposition of Insertion Sequences in the <i>dps</i>-Double-Knockout Mutant of <i>Deinococcus geothermalis</i>.

International journal of molecular sciences·2026
Same author

Inflammation and hypoalbuminemia mediate the association between CKD and contrast-associated AKI.

European journal of medical research·2026
Same author

Impact of cryotherapy on airway stenosis: In vivo, ex vivo, and in vitro studies.

Journal of thermal biology·2026

Related Experiment Video

Updated: May 27, 2026

Retroviral CRISPR/Cas9-Mediated Gene Targeting for the Study of Th17 Differentiation in Vitro
12:08

Retroviral CRISPR/Cas9-Mediated Gene Targeting for the Study of Th17 Differentiation in Vitro

Published on: November 15, 2024

Tristetraprolin down-regulates IL-17 through mRNA destabilization.

Hyun Hee Lee1, Nal Ae Yoon, Mai-Tram Vo

  • 1Department of Biological Sciences, University of Ulsan, Ulsan 680-749, Republic of Korea.

FEBS Letters
|December 6, 2011
PubMed
Summary

Tristetraprolin (TTP) inhibits interleukin 17 (IL-17) production in human T cells. TTP binds to IL-17 mRNA, promoting its decay and potentially controlling IL-17-mediated inflammation.

More Related Videos

Small RNA Transfection in Primary Human Th17 Cells by Next Generation Electroporation
10:15

Small RNA Transfection in Primary Human Th17 Cells by Next Generation Electroporation

Published on: April 13, 2017

Isolation and Th17 Differentiation of Na&iuml;ve CD4 T Lymphocytes
12:59

Isolation and Th17 Differentiation of Naïve CD4 T Lymphocytes

Published on: September 26, 2013

Related Experiment Videos

Last Updated: May 27, 2026

Retroviral CRISPR/Cas9-Mediated Gene Targeting for the Study of Th17 Differentiation in Vitro
12:08

Retroviral CRISPR/Cas9-Mediated Gene Targeting for the Study of Th17 Differentiation in Vitro

Published on: November 15, 2024

Small RNA Transfection in Primary Human Th17 Cells by Next Generation Electroporation
10:15

Small RNA Transfection in Primary Human Th17 Cells by Next Generation Electroporation

Published on: April 13, 2017

Isolation and Th17 Differentiation of Na&iuml;ve CD4 T Lymphocytes
12:59

Isolation and Th17 Differentiation of Naïve CD4 T Lymphocytes

Published on: September 26, 2013

Area of Science:

  • Immunology
  • Molecular Biology
  • Biochemistry

Background:

  • Interleukin 17 (IL-17) is implicated in chronic inflammatory disorders.
  • Mechanisms regulating IL-17 production in immune cells remain largely unknown.

Purpose of the Study:

  • To investigate the role of tristetraprolin (TTP) in regulating IL-17 production.
  • To elucidate the molecular mechanisms by which TTP affects IL-17 expression.

Main Methods:

  • Utilized human T cell lines for experiments.
  • Employed overexpression and siRNA inhibition techniques to study TTP function.
  • Analyzed IL-17 mRNA stability and TTP binding to the IL-17 3'UTR using molecular biology assays.

Main Results:

  • Overexpression of TTP led to decreased IL-17 production.
  • siRNA-mediated inhibition of TTP resulted in increased IL-17 production.
  • Tristetraprolin directly binds to the 3' untranslated region (3'UTR) of IL-17 mRNA, specifically between the fourth and seventh AU-rich elements (AREs).
  • TTP binding enhances the decay of IL-17 transcripts, thereby reducing IL-17 levels.

Conclusions:

  • Tristetraprolin acts as a negative regulator of IL-17 production in human T cells.
  • TTP controls IL-17-mediated inflammation through mRNA destabilization.
  • These findings identify TTP as a potential therapeutic target for IL-17-driven inflammatory conditions.