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Updated: May 27, 2026

Resin-Assisted Capture Coupled with Isobaric Tandem Mass Tag Labeling for Multiplexed Quantification of Protein Thiol Oxidation
Published on: June 21, 2021
Thioredoxin 1 is responsible for antibody disulfide reduction in CHO cell culture
Kristen L Koterba1, Trissa Borgschulte, Michael W Laird
1Late Stage Cell Culture, Genentech, Inc., 1 DNA Way, South San Francisco, CA 94080, USA. koterba.kristen@gene.com
Abstract:
During large-scale manufacturing of an IgG1 monoclonal antibody in Chinese hamster ovary (CHO) cells, reduction of the antibody's disulfide bonds was observed. We present evidence that mammalian thioredoxin 1 (TXN1) is the terminal enzyme responsible for this reduction event. We demonstrate a marked prevention of IgG1 disulfide bond reduction in a cell-density dependent manner by knocking down expression of TXN1 via lentivirus transduction of short hairpin RNA.
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