Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Regulation of Hematopoietic Stem Cells01:01

Regulation of Hematopoietic Stem Cells

All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...
Hematopoiesis01:21

Hematopoiesis

The process of blood cell formation is called hematopoiesis. Hematopoiesis starts early during development, on the seventh day of embryogenesis. This phase of hematopoiesis is called the primitive wave, wherein the extraembryonic yolk sac allows the production of erythroid cells and endothelial cells from a common precursor called hemangioblast. The erythroid cells provide oxygen to support the growth of the rapidly dividing embryo. Hemangioblasts later develop into hematopoietic stem cells or...
Lineage Commitment01:21

Lineage Commitment

Commitment is the  process whereby stem cells:
Multipotency of Hematopoietic Stem Cells01:19

Multipotency of Hematopoietic Stem Cells

The hematopoietic stem cells or HSCs are multipotent, meaning they can differentiate and give rise to all blood and immune cells. HSCs are maintained in the quiescent stage until an external stimulus initiates their differentiation. The multipotent HSCs exist as two heterogeneous populations, long-term repopulating cells (LTRC) and short-term repopulating cells (STRC). The two HSC populations have different surface markers or receptors and are classified based on quiescence and long-term...
Role of Hematopoietic Growth Factors01:28

Role of Hematopoietic Growth Factors

Hematopoietic growth factors are molecules that regulate the differentiation rate of hematopoietic stem cells (HSCs). Erythropoietin (EPO), primarily produced by the kidneys, plays a crucial role in erythrocyte production. When oxygen levels in the blood are low, EPO is released into the bloodstream, reaching the bone marrow, where it stimulates HSCs to differentiate and mature into erythrocytes, which are vital for oxygen transport.
Thrombopoietin (TPO), mainly released by the liver,...
Stem Cell Niche01:26

Stem Cell Niche

The stem cell niche is the dynamic microenvironment where stem cells reside. Inside these niches, the cells may remain undifferentiated, undergo high self-renewal, or become lineage-specific progenitors. Stem cells coexist with other niche cells, such as stromal cells. They also interact closely with the ECM. Cell-cell and cell-matrix communication occur via adhesion molecules or soluble factors that signal the stem cells and determine their fate. Stromal cells also provide survival signals to...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

ZNF512B safeguards genome integrity at regulatory regions to repress the SASP and inflammation.

Cell stem cell·2026
Same author

Proteomic signatures of the APOE ε4 and APOE ε2 genetic variants and Alzheimer's disease.

Nature aging·2026
Same author

Distinctive DNA sequence features define epigenetic longevity of inflammatory memory.

Science (New York, N.Y.)·2026
Same author

The DNA methylation landscape of naturally short-lived killifish.

Scientific reports·2026
Same author

Non-disruptive in vitro monitoring of cellular states with cell-free DNA methylation.

Genome biology·2026
Same author

Accelerating Medicines Partnership® Parkinson's Disease Proteomics: A Comprehensive Resource for Advancing Parkinson's Disease Research.

Movement disorders : official journal of the Movement Disorder Society·2026
Same journal

Mutational scanning reveals substrate-assisted autoregulation of the WNT destruction complex.

Nature genetics·2026
Same journal

Spatial transcriptomic analyses highlight distinct erythroid niches in mice and humans.

Nature genetics·2026
Same journal

Building up pangenome analysis block by block.

Nature genetics·2026
Same journal

Mutations in splicing factor gene U2AF1 rescue defective oncogene splicing in KRAS-mutant cancers.

Nature genetics·2026
Same journal

Assessing the effect of immune surveillance on clonal expansions in the blood.

Nature genetics·2026
Same journal

Improved heritability partitioning and enrichment analyses using summary statistics with graphREML.

Nature genetics·2026
See all related articles

Related Experiment Video

Updated: May 27, 2026

Identification of Key Factors Regulating Self-renewal and Differentiation in EML Hematopoietic Precursor Cells by RNA-sequencing Analysis
12:44

Identification of Key Factors Regulating Self-renewal and Differentiation in EML Hematopoietic Precursor Cells by RNA-sequencing Analysis

Published on: November 11, 2014

Dnmt3a is essential for hematopoietic stem cell differentiation.

Grant A Challen1, Deqiang Sun, Mira Jeong

  • 1Stem Cells and Regenerative Medicine Center, Baylor College of Medicine, Houston, Texas, USA.

Nature Genetics
|December 6, 2011
PubMed
Summary
This summary is machine-generated.

Loss of DNA methyltransferase Dnmt3a impairs hematopoietic stem cell (HSC) differentiation and expands HSC numbers. Dnmt3a is critical for epigenetic silencing of HSC genes, enabling efficient differentiation.

More Related Videos

Pan-myeloid Differentiation of Human Cord Blood Derived CD34+ Hematopoietic Stem and Progenitor Cells
10:25

Pan-myeloid Differentiation of Human Cord Blood Derived CD34+ Hematopoietic Stem and Progenitor Cells

Published on: August 9, 2019

Use of Hematopoietic Stem Cell Transplantation to Assess the Origin of Myelodysplastic Syndrome
06:39

Use of Hematopoietic Stem Cell Transplantation to Assess the Origin of Myelodysplastic Syndrome

Published on: October 3, 2018

Related Experiment Videos

Last Updated: May 27, 2026

Identification of Key Factors Regulating Self-renewal and Differentiation in EML Hematopoietic Precursor Cells by RNA-sequencing Analysis
12:44

Identification of Key Factors Regulating Self-renewal and Differentiation in EML Hematopoietic Precursor Cells by RNA-sequencing Analysis

Published on: November 11, 2014

Pan-myeloid Differentiation of Human Cord Blood Derived CD34+ Hematopoietic Stem and Progenitor Cells
10:25

Pan-myeloid Differentiation of Human Cord Blood Derived CD34+ Hematopoietic Stem and Progenitor Cells

Published on: August 9, 2019

Use of Hematopoietic Stem Cell Transplantation to Assess the Origin of Myelodysplastic Syndrome
06:39

Use of Hematopoietic Stem Cell Transplantation to Assess the Origin of Myelodysplastic Syndrome

Published on: October 3, 2018

Area of Science:

  • Epigenetics
  • Stem Cell Biology
  • Hematopoiesis

Background:

  • De novo DNA methyltransferases (Dnmt3a and Dnmt3b) are crucial for embryonic stem cell differentiation.
  • The role of Dnmt3a and Dnmt3b in somatic stem cells, particularly hematopoietic stem cells (HSCs), remains largely unknown.

Purpose of the Study:

  • To investigate the function of Dnmt3a in the maintenance and differentiation of HSCs.

Main Methods:

  • Conditional ablation of Dnmt3a in HSCs.
  • Serial transplantation assays to assess HSC function.
  • DNA methylation analysis (CpG island methylation).
  • Gene expression analysis of HSC multipotency and differentiation markers.

Main Results:

  • Dnmt3a loss progressively impairs HSC differentiation and leads to an expansion of HSC numbers in the bone marrow.
  • Dnmt3a-null HSCs exhibit altered DNA methylation patterns, including CpG island hypermethylation.
  • Dnmt3a deficiency results in upregulation of HSC multipotency genes and downregulation of differentiation factors.
  • Progeny of Dnmt3a-null HSCs show global hypomethylation and incomplete repression of HSC-specific genes.

Conclusions:

  • Dnmt3a is essential for the epigenetic silencing of genes regulating HSC function.
  • Dnmt3a plays a critical role in enabling efficient HSC differentiation by controlling gene expression through DNA methylation.